Dietary flaxseed administered post thoracic radiation treatment improves survival and mitigates radiation-induced pneumonopathy in mice.
Dietary flaxseed administered post thoracic radiation treatment improves survival and mitigates radiation-induced pneumonopathy in mice.
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DOI：
10.1186/1471-2407-11-269
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发表时间：
2011-06-24
期刊：
影响因子：
3.8
通讯作者：
Cengel KA
中科院分区：
文献类型：
--
作者：
Christofidou-Solomidou M;Tyagi S;Tan KS;Hagan S;Pietrofesa R;Dukes F;Arguiri E;Heitjan DF;Solomides CC;Cengel KA
亚麻籽（FS）是一种以其抗氧化和抗炎特性而闻名的膳食补充剂。肺组织的辐射暴露要么发生在治疗胸内恶性肿瘤时，要么发生在偶然情况下，例如在放射分散装置（RDD）爆炸后释放的吸入放射性同位素照射。这种暴露与肺部炎症、氧化组织损伤和不可逆肺纤维化有关。我们之前报道过饮食FS可以预防胸部x射线放射治疗（XRT）啮齿动物模型中的肺病。然而，亚麻籽在减轻辐照后辐射效应方面的治疗作用从未被评估过。我们在两次单独的实验（n = 15-25只小鼠/组）中评估了在单次13.5 Gy胸部放射治疗后0,2,4,6周给予小鼠（C57/BL6） 10%FS或等热量控制饮食的效果，并将其与在放射治疗前3周开始给予的既定辐射防护饮食进行比较。肺在xrt后4个月评估血氧水平、炎症和纤维化。饲喂0%FS的辐照小鼠4个月存活率为40%，而饲喂fs的辐照小鼠组的存活率为70-88%。此外，与喂食0%FS的小鼠相比，所有喂食fs的小鼠的纤维化都减少了。所有辐照fs组小鼠肺oh -脯氨酸含量为96.5±7.1 ~ 110.2±7.7 μg/ml (Mean±SEM)，而0%FS组为138±10.8 μg/ml。同时，在所有fs喂养组中，与辐射恶病质相关的支气管肺泡灌洗（BAL）蛋白和体重下降均显著降低。除了在XRT后延迟FS饮食4周和6周外，肺炎症细胞内流也显著减少。所有喂食fs的小鼠（无论是否辐照）与喂食0%FS的小鼠相比，血液氧合水平都保持较高。同样，BAL液中的多重细胞因子分析显示，fs喂养小鼠的特异性炎症细胞因子显著减少。xrt后给予FS的饮食通过减少肺纤维化、炎症、细胞因子分泌和肺损伤来减轻辐射效应，同时提高小鼠存活率。膳食补充FS可能是一种有用的辅助治疗，可以减轻吸入放射性同位素或偶然辐射暴露的个体的辐射不良反应。
Flaxseed (FS) is a dietary supplement known for its antioxidant and anti-inflammatory properties. Radiation exposure of lung tissues occurs either when given therapeutically to treat intrathoracic malignancies or incidentally, such as in the case of exposure from inhaled radioisotopes released after the detonation of a radiological dispersion devise (RDD). Such exposure is associated with pulmonary inflammation, oxidative tissue damage and irreversible lung fibrosis. We previously reported that dietary FS prevents pneumonopathy in a rodent model of thoracic X-ray radiation therapy (XRT). However, flaxseed's therapeutic usefulness in mitigating radiation effects post-exposure has never been evaluated. We evaluated the effects of a 10%FS or isocaloric control diet given to mice (C57/BL6) in 2 separate experiments (n = 15-25 mice/group) on 0, 2, 4, 6 weeks post a single dose 13.5 Gy thoracic XRT and compared it to an established radiation-protective diet given preventively, starting at 3 weeks prior to XRT. Lungs were evaluated four months post-XRT for blood oxygenation levels, inflammation and fibrosis. Irradiated mice fed a 0%FS diet had a 4-month survival rate of 40% as compared to 70-88% survival in irradiated FS-fed mouse groups. Additionally, all irradiated FS-fed mice had decreased fibrosis compared to those fed 0%FS. Lung OH-Proline content ranged from 96.5 ± 7.1 to 110.2 ± 7.7 μg/ml (Mean ± SEM) in all irradiated FS-fed mouse groups, as compared to 138 ± 10.8 μg/ml for mice on 0%FS. Concomitantly, bronchoalveolar lavage (BAL) protein and weight loss associated with radiation cachexia was significantly decreased in all FS-fed groups. Inflammatory cell influx to lungs also decreased significantly except when FS diet was delayed by 4 and 6 weeks post XRT. All FS-fed mice (irradiated or not), maintained a higher blood oxygenation level as compared to mice on 0%FS. Similarly, multiplex cytokine analysis in the BAL fluid revealed a significant decrease of specific inflammatory cytokines in FS-fed mice. Dietary FS given post-XRT mitigates radiation effects by decreasing pulmonary fibrosis, inflammation, cytokine secretion and lung damage while enhancing mouse survival. Dietary supplementation of FS may be a useful adjuvant treatment mitigating adverse effects of radiation in individuals exposed to inhaled radioisotopes or incidental radiation.
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影响因子：
7.4
作者：
Gauter-Fleckenstein, Benjamin;Fleckenstein, Katharina;Owzar, Kouros;Jiang, Chen;Reboucas, Julio S.;Batinic-Haberle, Ines;Vujaskovic, Zeljko
通讯作者：
Vujaskovic, Zeljko
影响因子：
28.5
作者：
Ao X;Zhao L;Davis MA;Lubman DM;Lawrence TS;Kong FM
通讯作者：
Kong FM
影响因子：
3.8
作者：
Dombroski, MJ;Fischbeck, PS
通讯作者：
Fischbeck, PS
影响因子：
2.6
作者：
Moulder, JE
通讯作者：
Moulder, JE
影响因子：
5.8
作者：
Citrin, Deborah;Cotrim, Ana P.;Mitchell, James B.
通讯作者：
Mitchell, James B.