Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice.
Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice.
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DOI：
10.1186/1756-8722-2-6
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发表时间：
2009-02-02
影响因子：
28.5
通讯作者：
Kong FM
中科院分区：
文献类型：
--
作者：
Ao X;Zhao L;Davis MA;Lubman DM;Lawrence TS;Kong FM
近年来的研究表明，多种细胞因子在放射性肺损伤中起重要作用。本研究旨在研究辐射后敏感小鼠（C57 BL/6）和抗性小鼠（C3 H）早期细胞因子诱导的差异。采用基于微球的多重细胞因子测定法分析12戈伊全肺照射后3、6、12、24 h至1周肺组织匀浆、支气管灌洗液（BAL）和血清中的22种细胞因子。大多数细胞因子在C57 BL/6和C3 H小鼠中具有相似的基线水平，但在辐射后显著不同。许多，包括粒细胞集落刺激因子（G-CSF），白细胞介素-6（IL-6），角质形成细胞衍生的趋化因子（KC）从两个菌株的标本中显着升高。在C57 BL/6和C3 H中，它们通常在3-6小时和6-12小时左右达到峰值。在6小时时，在肺组织中，G-CSF、IL-6和KC在C57 BL/6小鼠中分别增加6、8和11倍，在C3 H小鼠中分别增加4、3和3倍。6小时时，C57 BL/6 BAL液中的IL-6比C3 H BAL液高10倍。MCP-1、IP-10和IL-1α在肺组织和/或血清中也显示出一定的株间差异。对于相同的细胞因子和同一品系的小鼠，肺组织和BAL液水平之间（R2范围为0.46-0.99）以及血清和组织之间（R2范围为0.56-0.98）存在显著的线性相关性。辐射诱导肺纤维化敏感小鼠多种细胞因子的早期和更大的时间变化。血清和组织水平之间的正相关性表明，血液可用作组织的替代标记物。
Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensitive (C57BL/6) and resistant mice (C3H). Twenty-two cytokines in the lung tissue homogenates, bronchial lavage (BAL) fluids, and serum from 3, 6, 12, 24 hrs to 1 week after 12 Gy whole lung irradiation were profiled using a microsphere-based multiplexed cytokine assay. The majority of cytokines had similar baseline levels in C57BL/6 and C3H mice, but differed significantly after radiation. Many, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and keratinocyte-derived chemokine (KC) were elevated significantly in specimens from both strains. They usually peaked at about 3–6 hrs in C57BL/6 and 6–12 hrs in C3H. At 6 hrs in lung tissue, G-CSF, IL-6, and KC increased 6, 8, and 11 fold in C57BL/6 mice, 4, 3, and 3 fold in the C3H mice, respectively. IL-6 was 10-fold higher at 6 hrs in the C57BL/6 BAL fluid than the C3H BAL fluid. MCP-1, IP-10, and IL-1α also showed some differences between strains in the lung tissue and/or serum. For the same cytokine and within the same strain of mice, there were significant linear correlations between lung tissue and BAL fluid levels (R2 ranged 0.46–0.99) and between serum and tissue (R2 ranged 0.56–0.98). Radiation induced earlier and greater temporal changes in multiple cytokines in the pulmonary fibrosis sensitive mice. Positive correlation between serum and tissue levels suggests that blood may be used as a surrogate marker for tissue.
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影响因子：
2.6
作者：
Fedorocko, P;Egyed, A;Vacek, A
通讯作者：
Vacek, A
DOI：
10.1016/j.ijrobp.2005.05.032
发表时间：
2005-12-01
影响因子：
7
作者：
Hart, JP;Broadwater, G;Anscher, MS
通讯作者：
Anscher, MS
影响因子：
3.4
作者：
Johnston, CJ;Williams, JP;Finkelstein, JN
通讯作者：
Finkelstein, JN
DOI：
10.1016/s0360-3016(00)00482-x
发表时间：
2000-07-01
影响因子：
7
作者：
Rübe, CE;Uthe, D;Rübe, C
通讯作者：
Rübe, C
影响因子：
45.3
作者：
Arpin, D;Perol, D;Carrie, C
通讯作者：
Carrie, C