Actin dynamics affect mitochondrial quality control and aging in budding yeast.
Actin dynamics affect mitochondrial quality control and aging in budding yeast.
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DOI：
10.1016/j.cub.2013.10.022
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发表时间：
2013-12-02
期刊：
影响因子：
9.2
通讯作者：
Pon, Liza A.
中科院分区：
文献类型：
--
作者：
Higuchi, Ryo;Vevea, Jason D.;Swayne, Theresa C.;Chojnowski, Robert;Hill, Vanessa;Boldogh, Istvan R.;Pon, Liza A.
发芽酵母的肌动蛋白缆线是从芽尖或颈部延伸到母细胞顶端的一束束F-肌动蛋白，作为双向货物运输的轨迹，并经历从芽到母细胞的连续运动。这种运动称为逆行肌动蛋白电缆流(RACF)，类似于脂肪板层、生长锥体、免疫突触、树突棘和丝足的逆行肌动蛋白流动。在所有情况下，肌动蛋白流动都是由细胞皮质肌动蛋白聚合和组装的推动，以及肌球蛋白驱动的细胞内更深层次的拉力推动的。因此，为了从母体到芽体的运动和遗传，线粒体必须“逆流而上”，对抗RACF的相反力量。我们发现，增加RACF速率会导致芽继承的线粒体的适合度增加，并且线粒体适合度的增加会导致复制寿命的延长和细胞健康跨度的增加。Sirtuin Sir2是正常的RACF和线粒体健康所必需的，增加SIR2Δ细胞中的RACF比率可以增加线粒体的健康和细胞的健康寿命，但不会影响复制寿命。这些研究支持这样的模型，即在遗传过程中，RACF作为分离适合和不适合的线粒体的过滤器，控制着细胞的寿命和健康范围。它们还支持Sir2p在这些过程中发挥作用。
Actin cables of budding yeast are bundles of F-actin that extend from the bud tip or neck to the mother cell tip, serve as tracks for bidirectional cargo transport, and undergo continuous movement from buds towards mother cells. This movement, retrograde actin cable flow (RACF), is similar to retrograde actin flow in lamellipodia, growth cones, immunological synapses, dendritic spines and filopodia. In all cases, actin flow is driven by the push of actin polymerization and assembly at the cell cortex, and myosin-driven pulling forces deeper within the cell. Therefore, for movement and inheritance from mothers to buds, mitochondria must “swim upstream” against the opposing force of RACF. We find that increasing RACF rates results in increased fitness of mitochondria inherited by buds, and that the increase in mitochondrial fitness leads to extended replicative lifespan and increased cellular healthspan. The sirtuin SIR2 is required for normal RACF and mitochondrial fitness, and increasing RACF rates in sir2Δ cells increases mitochondrial fitness and cellular healthspan but does not affect replicative lifespan. These studies support the model that RACF serves as a filter for segregation of fit from less fit mitochondria during inheritance, which controls celllular lifespan and healthspan. They also support a role for Sir2p in these processes.
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DOI：
10.1016/j.cub.2011.10.019
发表时间：
2011-12-06
期刊：
Current biology : CB
影响因子：
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作者：
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期刊：
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DOI：
10.1073/pnas.022462899
发表时间：
2002-01-22
影响因子：
11.1
作者：
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