Thiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators.
Thiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators.
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DOI：
10.1002/1873-3468.14340
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发表时间：
2022-06
期刊：
影响因子：
3.5
通讯作者：
Puthiyaveetil, Sujith
中科院分区：
文献类型：
--
作者：
Ibrahim, Iskander M.;Rowden, Stephen J. L.;Cramer, William A.;Howe, Christopher J.;Puthiyaveetil, Sujith
蓝细菌采用双组分传感器响应调节器系统来监测和应对环境挑战。响应调节因子RpaA、RpaB、Rre 1和RppA是蓝藻生物钟功能和非生物胁迫适应的重要组成部分。已知RpaA、RpaB和Rre 1与铁氧还蛋白或硫氧还蛋白相互作用，提高了它们的巯基调节的可能性。在这里，我们报告了集胞藻PCC 6803 Rre 1，RpaA和RpaB在氧化条件下作为高级低聚物存在，并且还原的硫氧还蛋白A将它们转化为单体。我们进一步表明，这些响应调节剂含有氧化还原响应性半胱氨酸残基，其Em 7约为-300mV。这些研究结果表明，这些反应调节剂的活性的直接硫醇调制，独立于它们的同源传感器激酶。我们展示了蓝藻反应调节剂Rre 1，RpaA和RpaB的巯基介导的氧化还原调节，这些调节剂参与生物钟功能和非生物胁迫适应。氧化应激导致这些反应调节剂独立于磷酸化而寡聚化，为其DNA结合活性添加另一层调节。还原型硫氧还蛋白A将这些反应调节剂的高级低聚物转化为单体。
Cyanobacteria employ two‐component sensor‐response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in cyanobacteria. RpaA, RpaB and Rre1 are known to interact with ferredoxin or thioredoxin, raising the possibility of their thiol regulation. Here, we report that Synechocystis sp. PCC 6803 Rre1, RpaA and RpaB exist as higher‐order oligomers under oxidising conditions and that reduced thioredoxin A converts them to monomers. We further show that these response regulators contain redox‐responsive cysteine residues with an Em7 around −300 mV. These findings suggest a direct thiol modulation of the activity of these response regulators, independent of their cognate sensor kinases. We show thiol‐mediated redox regulation of cyanobacterial response regulators Rre1, RpaA and RpaB, which are involved in circadian clock function and abiotic stress acclimation. Oxidative stress causes these response regulators to oligomerise independently of phosphorylation, adding another layer of regulation to their DNA‐binding activity. Reduced thioredoxin A converts the higher‐order oligomers of these response regulators to monomers.
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影响因子：
27.5
作者：
Esther Perez-Perez, M.;Martin-Figueroa, Eugenio;Florencio, Francisco J.
通讯作者：
Florencio, Francisco J.
影响因子：
6.9
作者：
Mihara, Shoko;Sugiura, Kazunori;Hisabori, Toru
通讯作者：
Hisabori, Toru
DOI：
10.1073/pnas.1424632112
发表时间：
2015-02-17
影响因子：
11.1
作者：
Espinosa, Javier;Boyd, Joseph S.;Contreras, Asuncion
通讯作者：
Contreras, Asuncion
影响因子：
4.8
作者：
Paithoonrangsarid, K;Shumskaya, MA;Murata, N
通讯作者：
Murata, N
影响因子：
3.2
作者：
Li, H;Sherman, LA
通讯作者：
Sherman, LA