Growth hormone augments superoxide anion secretion of human neutrophils by binding to the prolactin receptor.
Growth hormone augments superoxide anion secretion of human neutrophils by binding to the prolactin receptor.
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生长激素通过与催乳素受体结合来增强人中性粒细胞的超氧阴离子分泌。
DOI：
10.1172/jci115605
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kelley,KW
中科院分区：
文献类型：
--
作者：
Fu,YK;Arkins,S;Fuh,G;Cunningham,BC;Wells,JA;Fong,S;Cronin,MJ;Dantzer,R;Kelley,KW
重组人生长激素（HuGH）和人催乳素（HuPRL），但不是牛或猪源GH，引发人中性粒细胞增强超氧阴离子（O2-）分泌。由于HuGH而不是其他物种的GH有效地结合HuPRL受体（HuPRL-R），我们使用了一组通过定点诱变产生的HuGH变体来鉴定人中性粒细胞上负责HuGH引发的受体。针对HuPRL-R的单克隆抗体（MAb）完全废除了与HuGH或HuPRL孵育的中性粒细胞的O2-分泌，而针对HuGH-R的MAb则没有效果。HuGH变体K172 A/F176 A对HuGH结合蛋白（BP）和HuPRL-BP的亲和力均降低，无法引发人中性粒细胞。这表明引发是由配体-受体相互作用启动的，其亲和力接近PRL和GH受体的亲和力。另一种HuGH变体K168 A/E174 A对HuPRL-BP的亲和力相对较低，但对HuGH-BP的亲和力略有增加，其致敏嗜中性粒细胞的能力大大降低。相比之下，HuGH变体E56 D/R64 M（其对HuPRL-BP具有与野生型HuGH相似的亲和力，但对HuGH-BP具有较低的亲和力）与野生型HuGH一样有效地引发嗜中性粒细胞。最后，HuGH与HuPRL-BP的结合而不是与HuGH-BP的结合已被证明是锌依赖性的，并且由HuGH引发的中性粒细胞也对锌有响应。总的来说，这些数据直接将HuGH与HuPRL-R的结合与人类靶细胞功能激活的一个方面联系起来。图片
Recombinant human growth hormone (HuGH) and human prolactin (HuPRL), but not GH of bovine or porcine origin, prime human neutrophils for enhanced superoxide anion (O2-) secretion. Since HuGH, but not GH of other species, effectively binds to the HuPRL receptor (HuPRL-R), we used a group of HuGH variants created by site-directed mutagenesis to identify the receptor on human neutrophils responsible for HuGH priming. A monoclonal antibody (MAb) directed against the HuPRL-R completely abrogated O2- secretion by neutrophils incubated with either HuGH or HuPRL, whereas a MAb to the HuGH-R had no effect. The HuGH variant K172A/F176A, which has reduced affinity for both the HuGH-binding protein (BP) and the HuPRL-BP, was unable to prime human neutrophils. This indicates that priming is initiated by a ligand-receptor interaction, the affinity of which is near that defined for receptors for PRL and GH. Another HuGH variant, K168A/E174A, which has relatively low affinity for the HuPRL-BP but slightly increased affinity for the HuGH-BP, had much reduced ability to prime neutrophils. In contrast, HuGH variant E56D/R64M, which has a similar affinity as wild-type HuGH for the HuPRL-BP but a lower affinity for the HuGH-BP, primed neutrophils as effectively as the wild-type HuGH. Finally, binding of HuGH to the HuPRL-BP but not to the HuGH-BP has been shown to be zinc dependent, and priming of neutrophils by HuGH was also responsive to zinc. Collectively, these data directly couple the binding of HuGH to the HuPRL-R with one aspect of functional activation of human target cells.Images
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垂体切除的大鼠依靠残留的催乳素生存。
影响因子：
4.8
作者：
É. Nagy;I. Bérczi
通讯作者：
I. Bérczi
垂体激素调节淋巴组织中的 c-myc 和 DNA 合成。
影响因子：
4.4
作者：
I. Bérczi;É. Nagy;S. M. de Toledo;R. Matusik;H. Friesen
通讯作者：
H. Friesen
补充锌可恢复老化脾细胞培养物中抗体的形成。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Winchurch,RA;Thomas,DJ;Adler,WH;Lindsay,TJ
通讯作者：
Lindsay,TJ
独特的胎盘催乳素受体：对胎儿生长的影响。
影响因子：
4.8
作者：
Freemark,M;Comer,M;Korner,G;Handwerger,S
通讯作者：
Handwerger,S
生长激素和胰岛素样生长因子-I 的新作用。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fu,YK;Arkins,S;Wang,BS;Kelley,KW
通讯作者：
Kelley,KW