Highly efficient microbial lipid synthesis from co-fermentation of enzymatic hydrolysate of sugarcane bagasse by a Trichosporon dermatis mutant
Highly efficient microbial lipid synthesis from co-fermentation of enzymatic hydrolysate of sugarcane bagasse by a Trichosporon dermatis mutant
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皮毛孢子突变体通过甘蔗渣酶解产物的共发酵高效合成微生物脂质
DOI：
10.1016/j.indcrop.2021.113975
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发表时间：
2021-11
影响因子：
5.9
通讯作者：
Peter James Strong
中科院分区：
文献类型：
--
作者：
Fubao Sun;Zhe Gu;Qiuli Zhou;Haiyan Sun;Jiancheng Luo;Zhongmei Liu;Shuxian Guo;Hongyan Ren;Zhanying Zhang;Peter James Strong
在这项研究中，通过顺序等离子体和化学诱变方法对一株产油酵母毛孢子皮炎进行了诱变。在不同规模和不同培养方式下，对甘蔗渣酶解物产脂最佳菌株(L7)进行了研究。结果表明，在摇瓶中，L7分批发酵含80g/L葡萄糖/木糖(质量比为2：1)的水解液，L7的菌体质量为26.7g/L，脂肪含量为14.0g/L；在5号L发酵罐中，L7分批发酵生产33.3g/L菌体，含18.1g/L油脂，补料分批发酵生产51.5g/L菌体，含31.3g/L油脂。本研究表明，L7是一种高效的T。使用从木质纤维素中提取的葡萄糖和木糖混合物生产脂肪的皮肤菌。
In this study, an oleaginous yeastTrichosporondermatiswas mutated by a sequential plasma and chemical mutagenesis approach. The best mutant (L7) was investigated to produce lipid from enzymatic hydrolysates derived from pretreated sugarcane bagasse at different scales and cultivation modes. The results showed that in shake flasks, L7 produced 26.7 g/L cell mass with 14.0 g/L lipid from the hydrolysate medium containing 80 g/L glucose/xylose (mass ratio of 2:1) in batch mode, and 34.9 g/L cell mass with 20.2 g/L lipid from the medium containing a total of 120 g/L glucose/xylose (2:1) in fed-batch mode, respectively. In a 5 L fermenter, L7 produced 33.3 g/L cell mass with 18.1 g/L lipid in batch mode and 51.5 g/L cell mass with 31.3 g/L lipid in fed-batch mode, respectively. This study demonstrates that L7 is highly efficientT. dermatisstrain to produce lipid using glucose and xylose mixture derived from lignocellulose.
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影响因子：
5
作者：
Sitepu IR;Jin M;Fernandez JE;da Costa Sousa L;Balan V;Boundy-Mills KL
通讯作者：
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影响因子：
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影响因子：
3.9
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