Salivary biomarkers for detection of systemic diseases.
Salivary biomarkers for detection of systemic diseases.
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DOI：
10.1371/journal.pone.0061356
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gustafsson A
中科院分区：
文献类型：
--
作者：
Rathnayake N;Akerman S;Klinge B;Lundegren N;Jansson H;Tryselius Y;Sorsa T;Gustafsson A
唾液中炎症生物标志物的分析可以为诊断不同的全身性疾病提供有吸引力的机会，特别是在流行病学调查中。本研究的目的是调查某些唾液生物标志物是否可用于检测常见的全身性疾病。随机选择了1000名居住在瑞典南部斯科讷县的成年人，邀请他们参加一项口腔健康的临床研究。451人参加了这项调查，其中51%为女性。所有参与者都被要求填写一份问卷，记录病史，进行临床检查，并收集刺激唾液样本。采用ELISA、IFMA或Luminex测定法测定唾液中IL-1β、IL-6、IL-8、TNF-α、溶菌酶、MMP-8和TIMP-1的浓度。唾液IL-8浓度被发现是两倍高的受试者谁有肿瘤疾病的经验。此外，IL-8水平也在肠道疾病患者中升高。MMP-8水平在心脏手术后或患有糖尿病、肌肉和关节疾病的患者的唾液中升高。在肌肉和关节疾病的受试者中，IL-1β、IL-8和MMP-8的水平以及MMP-8/TIMP-1的比率较高。唾液中的生物标志物有可能用于流行病学研究中的筛查目的。本研究中使用的相对非特异性的炎症标志物不能用于特定疾病的诊断，但可以被视为全身炎症增加的标志物。
Analysis of inflammatory biomarkers in saliva could offer an attractive opportunity for the diagnosis of different systemic conditions specifically in epidemiological surveys. The aim of this study was to investigate if certain salivary biomarkers could be used for detection of common systemic diseases. A randomly selected sample of 1000 adults living in Skåne, a county in the southern part of Sweden, was invited to participate in a clinical study of oral health. 451 individuals were enrolled in this investigation, 51% women. All participants were asked to fill out a questionnaire, history was taken, a clinical examination was made and stimulated saliva samples were collected. Salivary concentrations of IL-1β, -6, -8, TNF-α, lysozyme, MMP-8 and TIMP-1 were determined using ELISA, IFMA or Luminex assays. Salivary IL-8 concentration was found to be twice as high in subjects who had experience of tumour diseases. In addition, IL-8 levels were also elevated in patients with bowel disease. MMP-8 levels were elevated in saliva from patients after cardiac surgery or suffering from diabetes, and muscle and joint diseases. The levels of IL-1β, IL-8 and MMP-8, as well as the MMP-8/TIMP-1 ratio were higher in subjects with muscle and joint diseases. Biomarkers in saliva have the potential to be used for screening purposes in epidemiological studies. The relatively unspecific inflammatory markers used in this study can not be used for diagnosis of specific diseases but can be seen as markers for increased systemic inflammation.
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影响因子：
2.2
作者：
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作者：
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通讯作者：
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影响因子：
2
作者：
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通讯作者：
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