Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution.
Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution.
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DOI：
10.1186/1750-9378-9-22
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发表时间：
2014
影响因子：
3.7
通讯作者：
Brown DR
中科院分区：
文献类型：
--
作者：
Ermel A;Ramogola-Masire D;Zetola N;Tong Y;Qadadri B;Azar MM;Brown DR
宫颈癌是博茨瓦纳妇女因癌症死亡的主要原因。从生活在美国（n = 50）或博茨瓦纳（n = 171）的妇女中鉴定出福尔马林固定的浸润性宫颈癌标本的石蜡包埋块，从中提取DNA。对每个样本进行HPV类型和HIV的薄切片PCR。HPV类型和癌症中鉴定的类型组之间进行了比较。在美国92.0%的含有可扩增人类DNA的标本和博茨瓦纳79.5%的标本中鉴定出HPV DNA。来自美国的46例HPV阳性标本中有40例（87.0%）检出HPV 16，而来自博茨瓦纳的136例标本中有58例（42.7%）检出HPV 16（p < 0.001）。相比之下，非HPV 16/18型，所有A9种（HPV 16，31，33，35，52和58），非HPV 16 A9（HPV 31，33，35，52和58），HPV 18，所有A7型（18，39，45，59和68）型在博茨瓦纳标本中检出率显著更高。两组之间非HPV 18 A7类型的患病率没有显着差异。对于来自博茨瓦纳的标本，31.6%含有PCR可扩增的HIV序列，而美国标本中为3.9%。根据艾滋病毒状态对博茨瓦纳的样本进行分层，在艾滋病毒阳性标本中检测到HPV 31的频率明显更高。其他HPV类型和类型组之间没有显着差异艾滋病毒阳性和艾滋病毒阴性标本来自博茨瓦纳。这项研究表明，生活在美国或博茨瓦纳的妇女中发生的浸润性宫颈癌可能存在重要的HPV类型差异。除艾滋病毒外，其他因素也可能导致这些差异。
Cervical cancer is the primary cause of cancer-related deaths in women living in Botswana. Paraffin-embedded blocks of formalin-fixed invasive cervical cancer specimens were identified from women living in the U.S. (n = 50) or Botswana (n = 171) from which DNA was extracted. Thin-section PCR was performed on each sample for HPV types and HIV. Comparisons were made between HPV types and groups of types identified in cancers. HPV DNA was identified in 92.0% of specimens from the U.S. containing amplifiable human DNA, and 79.5% of specimens from Botswana. HPV 16 was detected in 40 of 46 HPV-positive specimens (87.0%) from the U.S. vs. 58 of 136 (42.7%) from Botswana (p < 0.001). In contrast, non-HPV 16/18 types, all A9 species (HPV16, 31, 33, 35, 52, and 58), non-HPV 16 A9 (HPV 31, 33, 35, 52, and 58), HPV 18, all A7 types (18, 39, 45, 59, and 68) types were detected significantly more often in specimens from Botswana. The prevalence of non-HPV 18 A7 types did not differ significantly between the two groups. For specimens from Botswana, 31.6% contained PCR-amplifiable HIV sequences, compared to 3.9% in U.S. specimens. Stratifying the samples from Botswana by HIV status, HPV 31 was detected significantly more often in HIV-positive specimens. Other HPV types and groups of types were not significantly different between HIV-positive and HIV-negative specimens from Botswana. This study demonstrates that there may be important HPV type differences in invasive cervical cancers occurring in women living in the United States or Botswana. Factors in addition to HIV may be driving these differences.
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