Prospects for the Structural Analysis of Biological Specimens by X-Ray Diffraction Imaging

Prospects for the Structural Analysis of Biological Specimens by X-Ray Diffraction Imaging
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X射线衍射成像生物样本结构分析的前景

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发表时间:
2018
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影响因子:
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通讯作者:
M. Nakasako
M. Nakasako
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作者:
M. Nakasako

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如第9、X射线衍射成像(XDI)具有重建生物样品的三维(3D)电子密度图的潜力。最近的XDI结构分析使我们有机会讨论未来的前景,在XDI实验中使用同步加速器X射线或X射线自由电子激光(XFEL)脉冲的生物标本的三维结构分析。在这里,我们考虑可实现的分辨率,考虑到同步辐射XDI实验中的辐射损伤和XFEL-XDI实验中的样品的异质性。
As described in Chap. 9, X-ray diffraction imaging (XDI) has the potential to reconstruct the three-dimensional (3D) electron density maps of biological specimens. The recent XDI structural analyses allow us the opportunity to discuss future prospects for the 3D structural analyses of biological specimens in XDI experiments using either synchrotron X-rays or X-ray free electron laser (XFEL) pulses. Here, we consider the achievable resolution, taking into account the radiation damage in synchrotron XDI experiments and the heterogeneity of specimens in XFEL-XDI experiments.
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