Expression analysis of RUS1 and construction of RUS1 plant expressing vector
Expression analysis of RUS1 and construction of RUS1 plant expressing vector
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RUS1表达分析及RUS1植物表达载体构建
DOI：
10.1007/s11703-010-0099-6
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发表时间：
2010-01
期刊：
影响因子：
--
通讯作者：
Zhiyong LI
中科院分区：
文献类型：
--
作者：
Jihong XING;Jingao DONG;Qiaoyun WENG;Zhiping DONG;Zhiyong LI
RUS1是从谷子中获得的抗病基因同源序列之一。 抗谷子单胞锈菌感染。采用Genome Walking方法获得RUS1启动子序列，全长675 bp。将RUS 1启动子与pCAMBIA 1300载体融合构建RUS 1：：GUS载体。GUS组织化学染色结果表明，启动子能够激活基因表达。通过PCR扩增获得RUS1基因（包括启动子序列），与pCAMBIA 1300载体融合，构建成pCAMBIA 1300：RUS1植物表达载体。该研究为RUS1基因的功能鉴定奠定了基础。
RUS1 was one of the disease resistance gene analogs obtained from Setaria italica Beauv. against Uromyces setariae-italicae infection. Promoter sequence of RUS1 was obtained by the method of Genome Walking, and its length was 675 bp. RUS1 promoter and pCAMBIA1300 vector were fused to construct RUS1::GUS vector. GUS histochemical staining result showed that promoter could activate gene expression. RUS1 gene (including the promoter sequence) was obtained through PCR amplification and then fused with pCAMBIA1300 vector to construct pCAMBIA1300:RUS1 plant expressing vector. The research laid a foundation for gene functional identification of RUS1.
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影响因子：
0.8
作者：
F. A. Azevedo;F. M. Filho;E. Schinor;Luis Gustavo de Paoli;B. J. Mendes;R. Harakava;D. Gabriel;Richard F. Lee
通讯作者：
F. A. Azevedo;F. M. Filho;E. Schinor;Luis Gustavo de Paoli;B. J. Mendes;R. Harakava;D. Gabriel;Richard F. Lee
影响因子：
3.3
作者：
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通讯作者：
C. Webb;T. Richter;N. Collins;Marie Nicolas;H. Trick;T. Pryor;S. Hulbert
影响因子：
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作者：
Chaoying He;Zhiyong Zhang;S. Chen
通讯作者：
Chaoying He;Zhiyong Zhang;S. Chen
影响因子：
13.8
作者：
Nagadhara, D;Ramesh, S;Rao, KV
通讯作者：
Rao, KV
影响因子：
1
作者：
Marcos Pileggi;A. Pereira;Joandrei dos Santos Silva;S. A. Pileggi;D. Verma
通讯作者：
Marcos Pileggi;A. Pereira;Joandrei dos Santos Silva;S. A. Pileggi;D. Verma