Changes in gene expression of individual matrix metalloproteinases differ in response to mechanical unloading of tendon fascicles in explant culture.
Changes in gene expression of individual matrix metalloproteinases differ in response to mechanical unloading of tendon fascicles in explant culture.
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DOI：
10.1002/jor.20650
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Derwin KA
中科院分区：
文献类型：
--
作者：
Leigh DR;Abreu EL;Derwin KA
肌腱和韧带的固定已被证明会导致材料性能快速而显着的下降。有人提出，导致人类肌腱断裂或疼痛的组织退化也可能与局灶性肌腱损伤后的机械卸载有关。因此，了解与机械卸载相关的重塑机制与人类的固定状态（例如铸造）、肌腱断裂的延迟修复以及潜在的过度使用损伤有关。这是第一项研究外植体模型中与组织收获和机械卸载培养相关的基因表达变化的时间过程的研究。收获大鼠尾腱束并置于空载培养物中长达 48 小时，然后使用 qRT-PCR 评估 12 个时间点的两个合成代谢基因和四个分解代谢基因的变化。我们的数据表明，I 型胶原、核心蛋白聚糖、组织蛋白酶 K 和 MMP2 基因表达对空载培养条件相对不敏感。然而，MMP3 和 MMP13 基因表达的变化是快速、剧烈、持续的，并且至少在卸载培养物的前 48 小时内发生变化。这些数据将有助于进一步阐明与肌腱机械卸载相关的机械性能损失的机制。
Immobilization of the tendonand ligament has beenshown to result in a rapid and significant decrease in material properties. It has been proposed that tissue degradation leading to tendon rupture or pain in humansmayalso be linked to mechanical unloading following focal tendon injury. Hence, understanding the remodeling mechanism associated with mechanical unloading has relevance for the human conditions of immobilization (e.g., casting), delayed repair of tendon ruptures, and potentially overuse injuries as well. This is the first study to investigate the time course of gene expression changes associated with tissue harvest and mechanical unloading culture in an explant model. Rat tail tendon fascicles were harvested and placed in culture unloaded for up to 48 h and then evaluated using qRT-PCR for changes in two anabolic and four catabolic genes at 12 time points. Our data demonstrates that Type I Collagen, Decorin, Cathepsin K, and MMP2 gene expression are relatively insensitive to unloaded culture conditions. However, changes in both MMP3 and MMP13 gene expression are rapid, dramatic, sustained, and changing during at least the first 48 h of unloaded culture. This data will help to further elucidate the mechanism for the loss of mechanical properties associated with mechanical unloading in tendon.
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