In utero exposure to ethanol affects postnatal development of T- and B-lymphocytes, but not natural killer cells.
In utero exposure to ethanol affects postnatal development of T- and B-lymphocytes, but not natural killer cells.
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在子宫内接触乙醇会影响 T 淋巴细胞和 B 淋巴细胞的出生后发育，但不会影响自然杀伤细胞。
DOI：
10.1111/j.1530-0277.1995.tb01487.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chervenak,R
中科院分区：
文献类型：
--
作者：
Wolcott,RM;Jennings,SR;Chervenak,R
本文研究了宫内乙醇暴露对C57BI/6J小鼠新生期淋巴细胞发育的影响。饲养小鼠，然后将雌性小鼠分成三组中的一组，即25%酒精衍生卡路里(EDC)组、配对喂养对照组或自由饮用实验室饲料组。在出生时，所有的后代都被交叉养育给喂过实验室食物的代孕母亲。每周取新生小鼠称重，每组取4只小鼠检测脾淋巴细胞的发育情况。在每一次采样中，所有三组的脾细胞总数都是相似的。用流式细胞仪检测T细胞、B细胞和自然杀伤(NK)细胞的数量。T细胞和NK细胞在三个饮食组之间没有显著差异。然而，在酒精暴露的动物中，在生命的前3周，B细胞总数减少。T细胞和B细胞的功能是通过评估对脂多糖、商陆有丝分裂原、植物血凝素和刀豆蛋白A的反应来确定的。在酒精暴露的动物中，对所有这四种有丝分裂原的反应显著降低，直到生命4-5周才恢复到对照水平。通过测定铬-51标记的YAC-1肿瘤靶细胞的杀伤率，发现乙醇暴露对NK细胞裂解功能的获得动力学没有显著影响。这些数据表明，出生前接触乙醇会导致免疫系统的部分但不是所有部分出现一过性免疫缺陷。
The effect of intrauterine exposure to ethanol on lymphocyte development in the neonatal period was studied in C57BI/6J mice. Mice were bred, and then the female mice were assigned to 1 of 3 diet groups, 25% ethanol‐derived calories (EDC), pair‐fed control, or ad libitum laboratory chow. At birth, all offspring were cross‐fostered to surrogate mothers who had been fed laboratory chow. At weekly intervals, the neonatal mice were weighed, and 4 mice from each group were used to assess the development of splenic lymphocytes. The total number of splenocytes was similar in all three groups at each sampling. The number of T‐cells, B‐cells, and natural killer (NK) cells was measured by flow cytometry. T‐cells and NK cells did not vary significantly among the three diet groups. However, the total number of B‐cells was decreased for the first 3 weeks of life in the ethanol‐exposed animals. The function of the T‐cells and B‐cells was determined by assessing the response to lipopolysaccharide, pokeweed mitogen, phytohemagglutinin, and concanavalin A. The response to all four mitogens was significantly reduced in the ethanol‐exposed animals and did not recover to control levels until 4–5 weeks of life. Ethanol exposure had no significant effect on the kinetics of acquisition of NK lytic function, as assessed by determining the killing of chromium‐51 labeled YAC‐1 tumor target cells. These data show that prenatal exposure to ethanol causes a transient immunodeficiency in some, but not all compartments of the immune system.
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影响因子：
11.2
作者：
Smithson,G;Wolcott,RM;Chervenak,R
通讯作者：
Chervenak,R
子宫内的酒精暴露会导致 T 细胞功能减弱以及大脑促肾上腺皮质激素释放因子和 ACTH 含量的改变。
DOI：
10.1111/j.1530-0277.1989.tb00350.x
发表时间：
1989
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Redei,E;Clark,WR;McGivern,RF
通讯作者：
McGivern,RF
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DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Colmenares,C;Lopez,C
通讯作者：
Lopez,C
MHC 抗原的靶细胞表达并不（始终）是自然杀伤细胞的关闭信号。
影响因子：
4.4
作者：
R. Chervenak;R. Wolcott
通讯作者：
R. Wolcott
影响因子：
4.4
作者：
S. Habu;H. Fukui;K. Shimamura;M. Kasai;Y. Nagai;K. Okumura;N. Tamaoki
通讯作者：
S. Habu;H. Fukui;K. Shimamura;M. Kasai;Y. Nagai;K. Okumura;N. Tamaoki