A non-sequence-specific DNA binding mode of RAG1 is inhibited by RAG2.
A non-sequence-specific DNA binding mode of RAG1 is inhibited by RAG2.
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DOI：
10.1016/j.jmb.2009.02.020
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发表时间：
2009-04-03
影响因子：
5.6
通讯作者：
Rodgers KK
中科院分区：
文献类型：
--
作者：
Zhao S;Gwyn LM;De P;Rodgers KK
The RAG1 and RAG2 proteins catalyze the site-specific DNA cleavage reactions in V(D)J recombination, the process that assembles antigen receptor genes from component gene segments during lymphocyte development. The first step towards the DNA cleavage reaction is the sequence-specific association of the RAG proteins with the conserved recombination signal sequence (RSS), which flanks each gene segment in the antigen receptor loci. Questions remain as to the contribution of each RAG protein to recognition of the RSS. For example, while RAG1 alone is capable of recognizing the conserved elements of the RSS, it is not clear if or how RAG2 may enhance sequence-specific associations with the RSS. To shed light on this issue, we examined the association of RAG1, with and without RAG2, to consensus RSS versus non-RSS substrates using fluorescence anisotropy and gel mobility shift assays. The results indicate that while RAG1 can recognize the RSS, the sequence-specific interaction at physiological conditions is masked by a high affinity non-sequence specific DNA-binding mode. Significantly, addition of RAG2 effectively suppressed the association of RAG1 with non-sequence specific DNA, resulting in a large differential in binding affinity for the RSS versus non-RSS sites. We conclude that this represents a major means by which RAG2 contributes to the initial recognition of the RSS, and that therefore association of RAG1 with RAG2 is required for effective interactions with the RSS in developing lymphocytes.
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影响因子：
4.8
作者：
Mo, XM;Bailin, T;Sadofsky, MJ
通讯作者：
Sadofsky, MJ
影响因子：
--
作者：
De, Pallabi;Zhao, Shuying;Gwyn, Lori M;Godderz, Leann J;Peak, Mandy M;Rodgers, Karla K
通讯作者：
Rodgers, Karla K
影响因子：
64.5
作者：
MCBLANE, JF;VANGENT, DC;OETTINGER, MA
通讯作者：
OETTINGER, MA
影响因子：
4.8
作者：
Ciubotaru, M;Ptaszek, LM;Schatz, DG
通讯作者：
Schatz, DG
影响因子：
12.4
作者：
Liu, Y.;Kulesz-Martin, M. F.
通讯作者：
Kulesz-Martin, M. F.