Co-registration of mouse cortical structures between in vivo and ex vivo images using tangential sectioning.
Co-registration of mouse cortical structures between in vivo and ex vivo images using tangential sectioning.
复制标题
使用切向切片在体内和离体图像之间配准小鼠皮质结构。
DOI：
10.1016/j.xpro.2022.101405
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Zhuang J
中科院分区：
文献类型：
--
作者：
Ouellette N;Turschak E;Zhuang J
在体内和离体成像之间的神经元结构共配准是研究哺乳动物大脑结构-功能对应的必要条件。在这里，我们描述了一种基于小鼠大脑切向切片的方案。该方案将体内双光子钙成像体积与离体共聚焦成像体积对齐，并在两个体积中记录相同的皮质结构。这种方法允许对这些实体的相应功能和结构进行详细分析。有关使用和执行本协议的完整详情，请参阅。切向切片促进了成像模式之间的共同配准体内双光子钙成像，随后是小鼠大脑的离体共聚焦成像，血管被标记为体内和离体图像对齐的基准标志体内到体外的共同配准使研究功能结构对应成为可能。作者注：开展任何实验方案都需要遵守当地机构的实验室安全和伦理指导方针。在体内和离体成像之间的神经元结构共配准是研究哺乳动物大脑结构-功能对应的必要条件。在这里，我们描述了一种基于小鼠大脑切向切片的方案。该方案将体内双光子钙成像体积与离体共聚焦成像体积对齐，并在两个体积中记录相同的皮质结构。这种方法允许对这些实体的相应功能和结构进行详细分析。
Co-registration of neuronal structures between in vivo and ex vivo imaging is necessary to study structure-function correspondence in the mammalian brain. Here we describe a protocol based on tangential sectioning of the mouse brain. This protocol aligns in vivo two-photon calcium imaging volumes with ex vivo confocal imaging volumes and registers the same cortical structures in both volumes. This approach allows detailed analysis of the corresponding function and structure of these entities. For complete details on the use and execution of this protocol, please refer to. Tangential sectioning facilitates co-registration between imaging modalities In vivo two-photon calcium imaging followed by ex vivo confocal imaging of mouse brain Blood vessels are labeled as fiducial landmarks for in vivo and ex vivo image alignment In vivo to ex vivo co-registration enables studying function-structure correspondence Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Co-registration of neuronal structures between in vivo and ex vivo imaging is necessary to study structure-function correspondence in the mammalian brain. Here we describe a protocol based on tangential sectioning of the mouse brain. This protocol aligns in vivo two-photon calcium imaging volumes with ex vivo confocal imaging volumes and registers the same cortical structures in both volumes. This approach allows detailed analysis of the corresponding function and structure of these entities.
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DOI：
10.3791/3564
发表时间：
2012-07-30
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gage, Gregory J;Kipke, Daryl R;Shain, William
通讯作者：
Shain, William
影响因子：
8.8
作者：
Zhuang J;Wang Y;Ouellette ND;Turschak EE;Larsen RS;Takasaki KT;Daigle TL;Tasic B;Waters J;Zeng H;Reid RC
通讯作者：
Reid RC
影响因子：
3
作者：
Bria A;Iannello G
通讯作者：
Iannello G
影响因子：
--
作者：
Harris, Julie A;Oh, Seung Wook;Zeng, Hongkui
通讯作者：
Zeng, Hongkui
影响因子：
3
作者：
Groblewski, P. A.;Sullivan, D.;Farrell, C.
通讯作者：
Farrell, C.