Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping.
Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping.
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DOI：
10.3791/56478
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发表时间：
2018-02-22
期刊：
影响因子：
--
通讯作者：
Furukawa T
中科院分区：
文献类型：
--
作者：
Ihara K;Sugiyama K;Takahashi K;Yamazoe M;Sasano T;Furukawa T
最近针对心房颤动（AF）的全基因组关联研究表明，心房的基因型和电生理表型之间存在很强的关联。这鼓励我们利用基因工程小鼠模型来阐明 AF 的机制。然而，由于小鼠心房体积小，很难评估其电生理特性。该协议描述了使用具有高时间和空间分辨率的光学测绘系统对 Langendorff 灌注小鼠心脏进行心房的电生理学评估。该光学测绘系统由双高速互补金属氧化物半导体相机和高倍物镜组装而成，可检测电压敏感染料和Ca2+指示剂的荧光。为了重点评估小鼠心房，光学测绘的面积为 2 mm × 2 mm 或 10 mm x 10 mm，分辨率为 100 × 100（20 µm/像素或 100 µm/像素），采样率最大可达 10 kHz（0.1 ms）。将 1-French 尺寸的四极电极起搏导管通过上腔静脉放入右心房，避免对心房造成任何机械损伤，并通过导管传递起搏刺激。电生理学研究是通过程序刺激进行的，包括恒定起搏、突发起搏和最多三重额外刺激起搏。在自发节律或起搏节律下，光学测绘分别记录右心房和左心房的动作电位持续时间、激活图、传导速度和 Ca2+ 瞬变。此外，程序刺激还决定了房性快速心律失常的诱发性。进行精确的激活映射以识别诱发房性快速心律失常期间心房中兴奋的传播。具有专门设置的光学测绘可以对小鼠病理模型中的心房进行彻底的电生理学评估。
Recent genome-wide association studies targeting atrial fibrillation (AF) have indicated a strong association between the genotype and electrophysiological phenotype in the atria. That encourages us to utilize a genetically-engineered mouse model to elucidate the mechanism of AF. However, it is difficult to evaluate the electrophysiological properties in murine atria due to their small size. This protocol describes the electrophysiological evaluation of atria using an optical mapping system with a high temporal and spatial resolution in Langendorff perfused murine hearts. The optical mapping system is assembled with dual high-speed complementary metal oxide semiconductor cameras and high magnification objective lenses, to detect the fluorescence of a voltage-sensitive dye and Ca2+ indicator. To focus on the assessment of murine atria, optical mapping is performed with an area of 2 mm × 2 mm or 10 mm x 10 mm, with a 100 × 100 resolution (20 µm/pixel or 100 µm/pixel) and sampling rate of up to 10 kHz (0.1 ms) at maximum. A 1-French size quadripolar electrode pacing catheter is placed into the right atrium through the superior vena cava avoiding any mechanical damage to the atrium, and pacing stimulation is delivered through the catheter. An electrophysiological study is performed with programmed stimulation including constant pacing, burst pacing, and up to triple extrastimuli pacing. Under a spontaneous or pacing rhythm, the optical mapping recorded the action potential duration, activation map, conduction velocity, and Ca2+ transient individually in the right and left atria. In addition, the programmed stimulation also determines the inducibility of atrial tachyarrhythmias. Precise activation mapping is performed to identify the propagation of the excitation in the atrium during an induced atrial tachyarrhythmia. Optical mapping with a specialized setting enables a thorough electrophysiological evaluation of the atrium in murine pathological models.
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影响因子：
37.8
作者：
Sinner MF;Tucker NR;Lunetta KL;Ozaki K;Smith JG;Trompet S;Bis JC;Lin H;Chung MK;Nielsen JB;Lubitz SA;Krijthe BP;Magnani JW;Ye J;Gollob MH;Tsunoda T;Müller-Nurasyid M;Lichtner P;Peters A;Dolmatova E;Kubo M;Smith JD;Psaty BM;Smith NL;Jukema JW;Chasman DI;Albert CM;Ebana Y;Furukawa T;Macfarlane PW;Harris TB;Darbar D;Dörr M;Holst AG;Svendsen JH;Hofman A;Uitterlinden AG;Gudnason V;Isobe M;Malik R;Dichgans M;Rosand J;Van Wagoner DR;METASTROKE Consortium;AFGen Consortium;Benjamin EJ;Milan DJ;Melander O;Heckbert SR;Ford I;Liu Y;Barnard J;Olesen MS;Stricker BH;Tanaka T;Kääb S;Ellinor PT
通讯作者：
Ellinor PT
影响因子：
3.5
作者：
Oishi, Sakiko;Sasano, Tetsuo;Furukawa, Tetsushi
通讯作者：
Furukawa, Tetsushi
影响因子：
30.8
作者：
Ellinor, Patrick T.;Lunetta, Kathryn L.;Albert, Christine M.;Glazer, Nicole L.;Ritchie, Marylyn D.;Smith, Albert V.;Arking, Dan E.;Mueller-Nurasyid, Martina;Krijthe, Bouwe P.;Lubitz, Steven A.;Bis, Joshua C.;Chung, Mina K.;Doerr, Marcus;Ozaki, Kouichi;Roberts, Jason D.;Smith, J. Gustav;Pfeufer, Arne;Sinner, Moritz F.;Lohman, Kurt;Ding, Jingzhong;Smith, Nicholas L.;Smith, Jonathan D.;Rienstra, Michiel;Rice, Kenneth M.;Van Wagoner, David R.;Magnani, Jared W.;Wakili, Reza;Clauss, Sebastian;Rotter, Jerome I.;Steinbeck, Gerhard;Launer, Lenore J.;Davies, Robert W.;Borkovich, Matthew;Harris, Tamara B.;Lin, Honghuang;Voelker, Uwe;Voelzke, Henry;Milan, David J.;Hofman, Albert;Boerwinkle, Eric;Chen, Lin Y.;Soliman, Elsayed Z.;Voight, Benjamin F.;Li, Guo;Chakravarti, Aravinda;Kubo, Michiaki;Tedrow, Usha B.;Rose, Lynda M.;Ridker, Paul M.;Conen, David;Tsunoda, Tatsuhiko;Furukawa, Tetsushi;Sotoodehnia, Nona;Xu, Siyan;Kamatani, Naoyuki;Levy, Daniel;Nakamura, Yusuke;Parvez, Babar;Mahida, Saagar;Furie, Karen L.;Rosand, Jonathan;Muhammad, Raafia;Psaty, Bruce M.;Meitinger, Thomas;Perz, Siegfried;Wichmann, H-Erich;Witteman, Jacqueline C. M.;Kao, W. H. Linda;Kathiresan, Sekar;Roden, Dan M.;Uitterlinden, Andre G.;Rivadeneira, Fernando;McKnight, Barbara;Sjogren, Marketa;Newman, Anne B.;Liu, Yongmei;Gollob, Michael H.;Melander, Olle;Tanaka, Toshihiro;Stricker, Bruno H. Ch;Felix, Stephan B.;Alonso, Alvaro;Darbar, Dawood;Barnard, John;Chasman, Daniel I.;Heckbert, Susan R.;Benjamin, Emelia J.;Gudnason, Vilmundur;Kaeaeb, Stefan
通讯作者：
Kaeaeb, Stefan
影响因子：
82.9
作者：
Sasano, Tetsuo;McDonald, Amy D.;Donahue, J. Kevin
通讯作者：
Donahue, J. Kevin
DOI：
10.1046/j.1540-8167.2003.02280.x
发表时间：
2003-03-01
影响因子：
2.7
作者：
Eijsbouts, SCM;Majidi, M;Allessie, MA
通讯作者：
Allessie, MA