KMS1 and KMS2, two plant endoplasmic reticulum proteins involved in the early secretory pathway.
KMS1 and KMS2, two plant endoplasmic reticulum proteins involved in the early secretory pathway.
复制标题
KMS1 和 KMS2，两种参与早期分泌途径的植物内质网蛋白。
DOI：
10.1111/j.1365-313x.2011.04522.x
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Wang P
我们已经确定了两个内质网（ER）相关的拟南芥蛋白，KMS 1和KMS 2，这是保守的大多数物种。KMS 1的荧光蛋白融合物定位于植物细胞中的ER，并且过表达诱导膜结构的形成，通过电子显微镜鉴定为ER螺旋。疏水性分析表明，KMS 1和KMS 2是具有6个跨膜结构域的完整膜蛋白。膜蛋白拓扑结构通过基于氧化还原的拓扑结构测定（ReTA）用氧化还原敏感的GFP进行评估，并通过蛋白酶保护测定进行确认。在ER的胞质侧发现跨膜结构域2和3之间的主要环结构域以及N-和C-末端。C末端二（三）赖氨酸基序参与KMS 1的恢复，缺失导致ER中GFP-KMS 1信号的减少。KMS 1/KMS 2截短的过表达扰乱了ER和高尔基体形态，当通过RNA干扰敲低KMS 1/KMS 2时也观察到类似的效果。显微镜和生化实验表明，KMS 1/KMS 2截短的表达抑制ER到高尔基体的蛋白质转运。
We have identified two endoplasmic reticulum (ER)‐associated Arabidopsis proteins, KMS1 and KMS2, which are conserved among most species. Fluorescent protein fusions of KMS1 localised to the ER in plant cells, and over‐expression induced the formation of a membrane structure, identified as ER whorls by electron microscopy. Hydrophobicity analysis suggested that KMS1 and KMS2 are integral membrane proteins bearing six transmembrane domains. Membrane protein topology was assessed by a redox‐based topology assay (ReTA) with redox‐sensitive GFP and confirmed by a protease protection assay. A major loop domain between transmembrane domains 2 and 3, plus the N‐ and C‐termini were found on the cytosolic side of the ER. A C‐terminal di(tri)‐lysine motif is involved in retrieval of KMS1 and deletion led to a reduction of the GFP–KMS1 signal in the ER. Over‐expression of KMS1/KMS2 truncations perturbed ER and Golgi morphology and similar effects were also seen when KMS1/KMS2 were knocked‐down by RNA interference. Microscopy and biochemical experiments suggested that expression of KMS1/KMS2 truncations inhibited ER to Golgi protein transport.
登录
查看更多内容
影响因子：
6.9
作者：
A. Osterrieder;E. Hummel;Claudine M Carvalho;C. Hawes
通讯作者：
A. Osterrieder;E. Hummel;Claudine M Carvalho;C. Hawes
影响因子：
12.3
作者：
Rabouille C;Kondylis V
通讯作者：
Kondylis V
影响因子：
3.5
作者：
Fujita, Eriko;Kouroku, Yoriko;Momoi, Takashi
通讯作者：
Momoi, Takashi
影响因子：
4
作者：
Kotzer, AM;Brandizzi, F;Hawes, C
通讯作者：
Hawes, C
影响因子：
4
作者：
Korkhov, Vladimir M.
通讯作者：
Korkhov, Vladimir M.