Infection of Human Endothelial Cells with Chlamydia pneumoniae Stimulates Transendothelial Migration of Neutrophils and Monocytes
Infection of Human Endothelial Cells with Chlamydia pneumoniae Stimulates Transendothelial Migration of Neutrophils and Monocytes
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肺炎衣原体感染人内皮细胞刺激中性粒细胞和单核细胞跨内皮迁移
DOI：
--
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发表时间：
1999
影响因子：
3.1
通讯作者：
J. Summersgill
中科院分区：
文献类型：
--
作者：
R. Molestina;Richardd . Miller;J. Ramirez;J. Summersgill
摘要 我们之前已经表明，肺炎衣原体的不同分离株在体外刺激来自受感染的人内皮细胞的趋化因子和粘附分子方面表现出异质性。在本研究中，我们检查了肺炎衣原体的不同分离株促进中性粒细胞和单核细胞跨内皮迁移的能力。人脐静脉内皮细胞（HUVEC）用低（<15）传代C感染。肺炎链球菌分离株 A-03、PS-32 和 BR-393 以及高 (>40) 传代分离株 BAL-16、TW-183 和 T-2634，并在感染 24 小时后测定中性粒细胞和单核细胞跨内皮迁移水平。与模拟感染的对照相比，观察到大多数肺炎衣原体分离株的中性粒细胞迁移显着增加（P < 0.001）。 TW-183 和 T-2634 后单核细胞迁移水平显着增加（P < 0.001）。在感染 HUVEC 之前，三种低传代分离株在 HEp-2 细胞培养物中连续传代（> 40 次）通常会促进更高水平的中性粒细胞和单核细胞跨内皮迁移。这些发现与在低传代和高传代 A-03、PS-32 和 BR-393 之间观察到的白细胞介素 8 (IL-8) 和单核细胞趋化蛋白 1 (MCP-1) 刺激程度的差异相一致。与C相反。在肺炎衣原体中，沙眼衣原体 L2 感染仅导致中性粒细胞跨内皮迁移略有增加，这与该物种缺乏可测量的 IL-8 水平相关。然而，尽管缺乏可测量的 MCP-1 刺激，但沙眼衣原体 L2 仍诱导显着水平的单核细胞迁移。沙眼衣原体血清型 A 和 E 也未能诱导 HUVEC 中 IL-8 和 MCP-1 的产生。这项研究的结果表明，肺炎衣原体的传代历史可能在人内皮细胞分离株之间观察到的刺激活性差异中发挥作用。此外，在该生物体和沙眼衣原体之间观察到的差异表明，内皮细胞中IL-8和MCP-1的上调可能是肺炎衣原体所独有的。
ABSTRACT We have previously shown that different isolates of Chlamydia pneumoniae display heterogeneity in the in vitro stimulation of chemokines and adhesion molecules from infected human endothelial cells. In the present study, we examined the ability of different isolates of C. pneumoniae to promote transendothelial migration of neutrophils and monocytes. Human umbilical vein endothelial cells (HUVEC) were infected with low (<15)-passageC. pneumoniae isolates A-03, PS-32, and BR-393 and high (>40)-passage isolates BAL-16, TW-183, and T-2634, and levels of neutrophil and monocyte transendothelial migration were determined following 24 h of infection. Compared to mock-infected controls, significant increases in neutrophil migration were observed in response to most C. pneumoniae isolates examined (P < 0.001). Levels of monocyte migration were significantly increased in response to TW-183 and T-2634 (P < 0.001). Serial passage (>40 times) of the three low-passage isolates in HEp-2 cell cultures prior to infection of HUVEC generally resulted in the promotion of higher levels of neutrophil and monocyte transendothelial migration. These findings were compatible with differences observed in the extent of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) stimulation between low- and high-passage A-03, PS-32, and BR-393. As opposed toC. pneumoniae, infection with C. trachomatis L2 caused only a slight increase in neutrophil transendothelial migration, which correlated with the lack of measurable IL-8 levels by this species. However, significant levels of monocyte migration were induced in response to C. trachomatis L2 despite a lack of measurable MCP-1 stimulation.C. trachomatis serovars A and E also failed to induce IL-8 and MCP-1 production in HUVEC. Results from this study indicate that the passage history of C. pneumoniae may play a role in the divergence of stimulatory activities observed among isolates in human endothelial cells. In addition, the differences observed between this organism and C. trachomatissuggest that the upregulation of IL-8 and MCP-1 in endothelial cells may be unique to C. pneumoniae.
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DOI：
10.1161/01.atv.13.10.1501
发表时间：
1993-10-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
KUO, CC;GOWN, AM;GRAYSTON, JT
通讯作者：
GRAYSTON, JT
DOI：
--
发表时间：
1985-04
期刊：
The American journal of pathology
影响因子：
--
作者：
C. Haslett;L. Guthrie;Marguerite M. Kopaniak;B. Richard;Johnston;P. Henson
通讯作者：
C. Haslett;L. Guthrie;Marguerite M. Kopaniak;B. Richard;Johnston;P. Henson
影响因子：
56.9
作者：
STRIETER, RM;KUNKEL, SL;MARKS, RM
通讯作者：
MARKS, RM
冠状动脉脂肪条纹和粥样斑块中肺炎衣原体的检测。
DOI：
--
发表时间：
1992
期刊：
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde
影响因子：
--
作者：
Shor,A;Kuo,CC;Patton,DL
通讯作者：
Patton,DL
DOI：
10.1016/0002-9343(93)90332-j
发表时间：
1993-11
期刊：
The American journal of medicine
影响因子：
--
作者：
S. Melnick;Eyal Shahar;A. Folsom;J. Grayston;P. Sorlie;San-Pin. Wang;M. Szklo
通讯作者：
S. Melnick;Eyal Shahar;A. Folsom;J. Grayston;P. Sorlie;San-Pin. Wang;M. Szklo