DNA methylation study of fetus genome through a genome-wide analysis.
DNA methylation study of fetus genome through a genome-wide analysis.
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通过全基因组分析对胎儿基因组进行 DNA 甲基化研究
DOI：
10.1186/1755-8794-7-18
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发表时间：
2014-04-15
影响因子：
2.7
通讯作者：
Zhu BF
中科院分区：
文献类型：
--
作者：
Wang HD;Hou QF;Guo QN;Li T;Wu D;Zhang XP;Chu Y;He M;Xiao H;Guo LJ;Yang K;Liao SX;Zhu BF
研究背景DNA甲基化是基因组的一种重要表观遗传修饰，它与胚胎发育、转录、染色质结构、X染色体失活、基因组印记和染色体稳定性等密切相关。与这些重要作用一致，DNA甲基化已被证明是脊椎动物早期胚胎发生所需的，并且对于调节控制细胞命运和分化的基因的时间和空间表达至关重要。进一步的研究表明，DNA甲基化异常与包括胚胎发育疾病在内的人类疾病有关。方法采用基因芯片技术（GeneChip ® Human Tiling 2.0R Array）对8对羊水和母血样本进行全基因组DNA甲基化分析，发现1个胎儿高甲基化DNA标记和4个胎儿低甲基化DNA标记。这些DNA标记均与胎儿生长发育的关键基因相关结论SH 2D 3C基因、EML 3基因、TRIM 71基因、HOXA 3基因和HOXA 5基因可作为胚胎发育相关性研究的生物标志物，病理性妊娠等。本研究为DNA甲基化在胚胎发育中的作用提供了新的基础性见解，病理性妊娠
BackgroundDNA methylation is a crucial epigenetic modification of the genome which is involved in embryonic development, transcription, chromatin structure, X chromosome inactivation, genomic imprinting and chromosome stability. Consistent with these important roles, DNA methylation has been demonstrated to be required for vertebrate early embryogenesis and essential for regulating temporal and spatial expression of genes controlling cell fate and differentiation. Further studies have shown that abnormal DNA methylation is associated with human diseases including the embryonic development diseases. We attempt to study the DNA methylation status of CpG islands in fetus related to fetus growth and development.MethodsGeneChip® Human Tiling 2.0R Array set is used for analysis of methylated DNA in a whole-genome wide in 8 pairs amniotic fluid and maternal blood DNA samples.ResultsWe found 1 fetus hypermethylation DNA markers and 4 fetus hypomethylation DNA markers though a Genome-wide analysis. These DNA markers all found to be associated with the critical genes for fetus growth and development (SH2D3C gene, EML3 gene, TRIM71 gene, HOXA3 gene and HOXA5 gene).ConclusionsThese genes can be used as a biomarker for association studying of embryonic development, pathological pregnancy and so on. The present study has provided new and fundamental insights into the roles that DNA methylation has in embryonic development and in the pathological pregnancy.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.3
作者：
Rai, Kunal;Nadauld, Lincoln D.;Jones, David A.
通讯作者：
Jones, David A.
斑马鱼发育过程中的全球和基因特异性DNA甲基化变化。
DOI：
10.1016/j.cbpb.2013.07.007
发表时间：
2013-09
影响因子：
2.2
作者：
Fang, Xiefan;Corrales, Jone;Thornton, Cammi;Scheffler, Brian E.;Willett, Kristine L.
通讯作者：
Willett, Kristine L.
影响因子：
23.9
作者：
Trowbridge JJ;Snow JW;Kim J;Orkin SH
通讯作者：
Orkin SH
影响因子：
16.6
作者：
Lambrot, R.;Xu, C.;Saint-Phar, S.;Chountalos, G.;Cohen, T.;Paquet, M.;Suderman, M.;Hallett, M.;Kimmins, S.
通讯作者：
Kimmins, S.