Global and gene specific DNA methylation changes during zebrafish development.
Global and gene specific DNA methylation changes during zebrafish development.
复制标题
斑马鱼发育过程中的全球和基因特异性DNA甲基化变化。
DOI：
10.1016/j.cbpb.2013.07.007
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发表时间：
2013-09
影响因子：
2.2
通讯作者：
Willett, Kristine L.
中科院分区：
文献类型：
--
作者：
Fang, Xiefan;Corrales, Jone;Thornton, Cammi;Scheffler, Brian E.;Willett, Kristine L.
DNA甲基化在生物体的整个生命过程中都是动态的。以前的研究主要集中在非常早期胚胎发生期间的DNA甲基化变化。本研究比较了斑马鱼（Danio rerio）胚胎、幼鱼和成鱼肝脏中DNA的整体甲基化和基因特异性甲基化。在受精后2至4.3小时（hpf）的斑马鱼胚胎中，细胞嘧啶甲基化的百分比较低，而在6 hpf以上的斑马鱼中，细胞嘧啶甲基化的百分比一直较高。实时荧光定量PCR （qPCR）结果显示，胚胎发生早期DNA甲基转移酶1 (dnmt1) mRNA表达较高，甘氨酸n -甲基转移酶（gnmt） mRNA表达较低。通过研究vasa、ras -关联结构域家族成员1 （rassf1）、端粒酶逆转录酶（tert）、c-jun和c-myca 5个发育重要基因的甲基化模式和基因表达，我们发现DNA甲基化模式变化的时间是基因特异性的，基因表达的变化与DNA甲基化模式并不一定相关。
DNA methylation is dynamic through the life of an organism. Previous studies have primarily focused on DNA methylation changes during very early embryogenesis. In this study, global and gene specific DNA methylation in zebrafish (Danio rerio) embryos, larvae and adult liver were compared. The percent methylation of cytosines was low in 2 to 4.3 hours post fertilization (hpf) zebrafish embryos and was consistently higher in zebrafish older than 6 hpf. Furthermore, quantitative real-time PCR (qPCR) results showed relatively high DNA methyltransferase 1 (dnmt1) and low glycine N-methyltransferase (gnmt) mRNA expression in early embryogenesis. By studying methylation patterns and gene expression of five developmentally important genes, namely vasa, Ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun and c-myca, we found that the timing of changes in DNA methylation patterns was gene specific, and changes in gene expression were not necessarily correlated with the DNA methylation patterns.
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影响因子：
14.9
作者：
Bauer AP;Leikam D;Krinner S;Notka F;Ludwig C;Längst G;Wagner R
通讯作者：
Wagner R
影响因子：
3.7
作者：
Anchelin M;Murcia L;Alcaraz-Pérez F;García-Navarro EM;Cayuela ML
通讯作者：
Cayuela ML
影响因子：
11.8
作者：
Aniagu, Stanley O.;Williams, Tim D.;Chipman, J. Kevin
通讯作者：
Chipman, J. Kevin
影响因子：
4.3
作者：
Fang X;Thornton C;Scheffler BE;Willett KL
通讯作者：
Willett KL
影响因子：
7.8
作者：
Eferl, R;Sibilia, M;Hilberg, F;Fuchsbichler, A;Kufferath, I;Guertl, B;Zenz, R;Wagner, E F;Zatloukal, K
通讯作者：
Zatloukal, K