Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes.
Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes.
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DOI：
10.1038/ncomms3889
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发表时间：
2013
影响因子：
16.6
通讯作者：
Kimmins, S.
中科院分区：
文献类型：
--
作者：
Lambrot, R.;Xu, C.;Saint-Phar, S.;Chountalos, G.;Cohen, T.;Paquet, M.;Suderman, M.;Hallett, M.;Kimmins, S.
流行病学研究表明，父亲的饮食会影响后代的健康。一种被提出的父系环境信息传递机制是通过精子表观基因组。表观基因组包括遗传信息，如DNA甲基化。我们假设饮食中甲基供体的供应会改变精子的表观遗传重编程。在这里，我们喂养雄性小鼠无论是叶酸缺乏或叶酸充足的饮食在整个生命。父亲叶酸缺乏与后代出生缺陷增加有关，包括颅面和肌肉骨骼畸形。全基因组DNA甲基化分析和随后的功能分析鉴定了精子中与发育、慢性疾病如癌症、糖尿病、自闭症和精神分裂症有关的基因的差异甲基化。虽然后代胎盘中有超过300个基因差异表达，但只有两个对应于精子中具有差异甲基化的基因。该模型表明表观遗传传递可能涉及精子组蛋白H3甲基化或DNA甲基化，并且足够的父亲膳食叶酸对后代健康至关重要。 在大鼠和小鼠中，父亲的饮食已被证明会影响后代的代谢。Lambrot等报道了叶酸缺乏饮食的雄性小鼠精子中DNA和组蛋白甲基化的变化，并观察了其后代的发育缺陷。
Epidemiological studies suggest that a father’s diet can influence offspring health. A proposed mechanism for paternal transmission of environmental information is via the sperm epigenome. The epigenome includes heritable information such as DNA methylation. We hypothesize that the dietary supply of methyl donors will alter epigenetic reprogramming in sperm. Here we feed male mice either a folate-deficient or folate-sufficient diet throughout life. Paternal folate deficiency is associated with increased birth defects in the offspring, which include craniofacial and musculoskeletal malformations. Genome-wide DNA methylation analysis and the subsequent functional analysis identify differential methylation in sperm of genes implicated in development, chronic diseases such as cancer, diabetes, autism and schizophrenia. While >300 genes are differentially expressed in offspring placenta, only two correspond to genes with differential methylation in sperm. This model suggests epigenetic transmission may involve sperm histone H3 methylation or DNA methylation and that adequate paternal dietary folate is essential for offspring health. Paternal diet has been shown to influence the metabolism of offspring in rats and mice. Here Lambrot et al. report DNA and histone methylation changes in the sperm of male mice on a folate-deficient diet and observe developmental defects in their offspring.
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DOI：
10.1126/science.1229277
发表时间：
2013-01-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hackett JA;Sengupta R;Zylicz JJ;Murakami K;Lee C;Down TA;Surani MA
通讯作者：
Surani MA
影响因子：
64.5
作者：
Carone BR;Fauquier L;Habib N;Shea JM;Hart CE;Li R;Bock C;Li C;Gu H;Zamore PD;Meissner A;Weng Z;Hofmann HA;Friedman N;Rando OJ
通讯作者：
Rando OJ
影响因子：
16.8
作者：
Brykczynska, Urszula;Hisano, Mizue;Peters, Antoine H. F. M.
通讯作者：
Peters, Antoine H. F. M.
影响因子：
4
作者：
Delaval, K;Feil, R
通讯作者：
Feil, R
DOI：
10.1073/pnas.94.7.3290
发表时间：
1997-04-01
影响因子：
11.1
作者：
Blount, BC;Mack, MM;Ames, BN
通讯作者：
Ames, BN