Polymer Nanoparticle Engineering for Podocyte Repair: From in Vitro Models to New Nanotherapeutics in Kidney Diseases.
Polymer Nanoparticle Engineering for Podocyte Repair: From in Vitro Models to New Nanotherapeutics in Kidney Diseases.
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DOI：
10.1021/acsomega.6b00423
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发表时间：
2017-02-28
期刊：
影响因子：
4.1
通讯作者：
Cellesi F
中科院分区：
文献类型：
--
作者：
Colombo C;Li M;Watanabe S;Messa P;Edefonti A;Montini G;Moscatelli D;Rastaldi MP;Cellesi F
肾足细胞是参与蛋白尿疾病发生和/或进展的高度分化的分支肾小球细胞，特异性靶向治疗可能成为预防慢性肾脏疾病的最佳策略。有了这个目标，我们开发了一个库的工程聚合物纳米粒子（NP）的可调大小和表面特性，并评估其与足细胞的相互作用。首先评估NP细胞毒性、摄取和对足细胞的细胞骨架效应。在这些数据的基础上，将地塞米松纳米递送加载到所选择的生物相容性NP中成功地修复了受损的足细胞。最后，内皮细胞和足细胞共培养的三维体外系统被用作模拟NP与肾小球细胞相互作用和肾脏滤过屏障修复机制的新工具。
Specific therapeutic targeting of kidney podocytes, the highly differentiated ramified glomerular cells involved in the onset and/or progression of proteinuric diseases, could become the optimal strategy for preventing chronic kidney disease. With this aim, we developed a library of engineered polymeric nanoparticles (NPs) of tuneable size and surface properties and evaluated their interaction with podocytes. NP cytotoxicity, uptake, and cytoskeletal effects on podocytes were first assessed. On the basis of these data, nanodelivery of dexamethasone loaded into selected biocompatible NPs was successful in repairing damaged podocytes. Finally, a three-dimensional in vitro system of co-culture of endothelial cells and podocytes was exploited as a new tool for mimicking the mechanisms of NP interaction with glomerular cells and the repair of the kidney filtration barrier.
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地塞米松的块共聚物纳米颗粒会诱导白血病细胞死亡并增强治疗功效：一种新的小儿纳米医学应用。
影响因子：
4.9
作者：
Krishnan V;Xu X;Barwe SP;Yang X;Czymmek K;Waldman SA;Mason RW;Jia X;Rajasekaran AK
通讯作者：
Rajasekaran AK
影响因子：
4.6
作者：
Li, Min;Corbelli, Alessandro;Rastaldi, Maria Pia
通讯作者：
Rastaldi, Maria Pia
影响因子：
8
作者：
Fröhlich E
通讯作者：
Fröhlich E
影响因子：
5.8
作者：
Ferrari, Raffaele;Colombo, Claudio;Moscatelli, Davide
通讯作者：
Moscatelli, Davide
影响因子：
4.9
作者：
Ferrari, Raffaele;Talamini, Laura;Moscatelli, Davide
通讯作者：
Moscatelli, Davide