First case of Aeromonas schubertii infection in brackish water wild Nile tilapia, Oreochromis niloticus, in China
First case of Aeromonas schubertii infection in brackish water wild Nile tilapia, Oreochromis niloticus, in China
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中国首例咸水野生尼罗罗非鱼Oreochromis niloticus感染舒伯气单胞菌
DOI：
10.1016/j.aquaculture.2018.11.036
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发表时间：
2019-02
期刊：
影响因子：
4.5
通讯作者：
Zhou Yongcan
中科院分区：
文献类型：
--
作者：
Ren Zhuling;Cai Yan;Wang Shifeng;Liu Shubin;Li An;Xiong Youfei;Tang Jiaqing;Sun Yun;Guo Weiliang;Zhou Yongcan
为鉴定海口某泻湖罗非鱼(Oreochromis Niloticus)死亡的病原菌，从死鱼中分离到4株罗非鱼菌株，分别命名为ASL-1、ASB-2、ASB-3和ASS-4。革兰氏阴性、能动、矮小、杆状。对其16S rRNA基因和看家基因dna J、ela和gyrB的序列分析表明，这4株菌与舒伯蒂气单胞菌一致。毒力测定表明，ASS-4毒力最强，7 d的致死量(LD50)为4.91 × 104CFU/g鱼。注射。不同的ASS-4感染途径导致不同的死亡率。两次腹腔注射后均出现急性死亡。和肌内(I.S.)打针。口腔暴露后的死亡率很低，浸泡后没有观察到死亡。这表明OFA是通过自然感染途径传播的。罗非鱼可能是通过受损的体表和/或消化道。被挑战的鱼的组织病理学包括肝脏显示空泡化，脾出血和大脑毛细血管肿胀。药敏试验表明，ASS-4对诺氟沙星和利福平敏感，对10种抗生素耐药。这是第一次有这样的报道。Schubertii感染了咸水野鱼。这也是第一次对FA可能的进入路线进行调查。苏伯丁鱼。这些结果将有助于阐明FA的致病性。舒伯蒂安将有助于预防和控制疟疾。罗非鱼的舒伯氏菌感染。
To identify and characterize the etiological agent causing tilapia (Oreochromis niloticus) mortality in a lagoon located in Haikou, China, four isolates, designated ASL-1, ASB-2, ASB-3 and ASS-4, were recovered from moribund fish. They were gram-negative, motile, short and rod-shaped. Sequencing of the 16S rRNA gene, as well as of the housekeeping genes-dnaJ,elastase(ela) andgyrase subunit beta(gyrB), strongly indicated that the four isolates were identical toAeronomas schubertii.A virulence test showed that ASS-4 was the most virulent, and the lethal dose (LD50) of 7 days was 4.91 × 104CFU/g fish by intraperitoneal (i.p.) injection. Different infection routes with ASS-4 induced different mortality rates. Acute mortalities were present after both i.p. and intramuscular (i.s.) injections. Very low mortalities were observed after oral exposure, and no mortality was observed after immersion. This suggested that the natural infection routes ofA. schubertiiin tilapia might be through damaged body surface and/or digestive tract. The histopathology of challenged fish included livers showing vacuolation, spleens with hemorrhaging and brains with swelling capillaries. Antibiotic sensitivity testing indicated that ASS-4 was susceptible to norfloxacin and rifampicin but resistant to 10 different antibiotics. This is the first report thatA. schubertiiinfected brackish water wild fish. This is also the first investigation of the possible entry routes ofA. schubertiiin fish. These results will shed light on the pathogenicity ofA. schubertiiand will help prevent and controlA. schubertiiinfection in tilapia.
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DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
Ali Aberoum;H. Jooyandeh
通讯作者：
Ali Aberoum;H. Jooyandeh
DOI：
10.4314/ajfand.v11i7
发表时间：
2011-12
影响因子：
--
作者：
A. Akinwale
通讯作者：
A. Akinwale
影响因子：
3.3
作者：
C. Werckenthin;Annerose Gey;R. Straubinger;S. Poppert
通讯作者：
C. Werckenthin;Annerose Gey;R. Straubinger;S. Poppert
DOI：
--
发表时间：
1980-12
期刊：
New York state journal of medicine
影响因子：
--
作者：
S. Dhalla;J. T. Flynn
通讯作者：
S. Dhalla;J. T. Flynn
影响因子：
4.5
作者：
J. Kumari;P. Sahoo
通讯作者：
J. Kumari;P. Sahoo