Simultaneous determination of multiple phthalate metabolites and bisphenol-A in human urine by liquid chromatography-tandem mass spectrometry.
Simultaneous determination of multiple phthalate metabolites and bisphenol-A in human urine by liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/j.jchromb.2012.07.022
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发表时间：
2012-09-01
影响因子：
3
通讯作者：
Lu, Chensheng
中科院分区：
文献类型：
--
作者：
Chen, Mei;Tao, Lin;Collins, Erin M.;Austin, Christine;Lu, Chensheng
邻苯二甲酸酯和双酚A是常见消费品中广泛使用的环境内分泌干扰物。由于邻苯二甲酸酯的抗雄激素活性和双酚A的雌激素活性相关的潜在不良影响，人们越来越关注邻苯二甲酸酯和双酚A对人类的暴露。在评估环境中邻苯二甲酸酯和双酚A的暴露时，有必要建立一种有效的分析方法来定量测定人体中邻苯二甲酸酯代谢物和双酚A的痕量浓度。在这项研究中，我们建立并验证了一种准确、灵敏和可靠的LC-MS/MS方法来同时定量人尿中的5种邻苯二甲酸单酯代谢物，包括邻苯二甲酸单甲酯、邻苯二甲酸单乙酯、邻苯二甲酸单丁酯、邻苯二甲酸单苄酯、邻苯二甲酸单乙基己酯和双酚A。采用全自动离线固相萃取法，从2 0 0μL尿样中提取邻苯二甲酸酯类代谢物和双酚A及其同位素标记内标。采用LC-MS/MS负电喷雾电离多反应监测模式进行定量测定。邻苯二甲酸单甲酯、邻苯二甲酸单乙酯、邻苯二甲酸单苄酯和双酚A的最低定量限为0.3 ng/ml，邻苯二甲酸单丁酯和邻苯二甲酸单异辛酯的最低定量限为1 ng/ml。精密度和准确度均在可接受的15%范围内。这种经过验证的方法已成功地用于评估邻苯二甲酸盐和双酚A对人体的暴露。
Phthalates and bisphenol A are environmental endocrine-disrupting chemicals used widely in common consumer products. There is increasing concern about human exposure to phthalates and bisphenol A due to the potential adverse effects related to the anti-androgenic activity of phthalates and estrogenic activity of bisphenol A. In assessing environmental exposure to phthalates and bisphenol A, it is essential to have a validated analytical method that can quantify trace concentrations of phthalate metabolites and bisphenol A in humans. In this study, we developed and validated an accurate, sensitive, and robust LC-MS/MS method to simultaneously quantify 5 phthalate monoester metabolites, including mono-methyl phthalate, mono-ethyl phthalate, mono-butyl phthalate, mono-benzyl phthalate, mono-2-ethylhexyl phthalate, and bisphenol A in human urine. In this method, the phthalate metabolites and bisphenol A, along with their isotope labeled internal standards, were extracted from 200 μl of human urine using automated off-line solid phase extraction. The analytes were quantitatively determined using LC-MS/MS operated in negative electrospray ionization multiple reaction-monitoring mode. The limit of quantification was 0.3 ng/ml for mono-methyl phthalate, mono-ethyl phthalate, mono-benzyl phthalate and bisphenol A, and 1 ng/ml for mono-butyl phthalate and mono-2-ethylhexyl phthalate. The precision and accuracy were well within the acceptable 15% range. This validated method has been used successfully in assessing exposure to phthalates and bisphenol A in humans.
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影响因子：
7.4
作者：
Kato, K;Silva, MJ;Calafat, AM
通讯作者：
Calafat, AM
邻苯二甲酸盐和双苯酚A浓度与血清甲状腺甲状腺措施之间的关系，来自国家健康与营养检查调查（NHANES）2007-2008之间的关系。
影响因子：
10.4
作者：
Meeker JD;Ferguson KK
通讯作者：
Ferguson KK
DOI：
10.1016/j.jchromb.2007.10.023
发表时间：
2007-12-01
影响因子：
3
作者：
Silva, Manori J.;Samandar, Ella;Calafat, Antonia M.
通讯作者：
Calafat, Antonia M.
影响因子：
3
作者：
Takashima, Kayoko;Ito, Yuki;Nakajima, Tamie
通讯作者：
Nakajima, Tamie
影响因子：
8.3
作者：
Vandentorren, Stephanie;Zeman, Florence;Leridon, Henri
通讯作者：
Leridon, Henri