Chondrocytes and meniscal fibrochondrocytes differentially process aggrecan during de novo extracellular matrix assembly.
Chondrocytes and meniscal fibrochondrocytes differentially process aggrecan during de novo extracellular matrix assembly.
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软骨细胞和半月板纤维软骨细胞在从头细胞外基质组装过程中差异性地处理聚集蛋白聚糖。
DOI：
10.1089/ten.tea.2008.0106
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发表时间：
2009
影响因子：
--
通讯作者：
Levenston,MarcE
中科院分区：
文献类型：
--
作者：
Wilson,ChristopherG;Nishimuta,JamesF;Levenston,MarcE
聚集蛋白聚糖是一种细胞外基质分子，其有助于关节软骨和关节纤维软骨的机械特性，但聚集蛋白聚糖在这些组织中的丰度和加工是不同的。本研究的目的是比较软骨细胞和软骨纤维软骨细胞在组织外植体和细胞琼脂糖结构中的聚集蛋白聚糖加工模式。检测转化生长因子-β 1（TGF-β1）刺激对聚集蛋白聚糖沉积和加工的影响，并测量构建体的力学性能。纤维软骨细胞合成和保留较少的蛋白多糖比软骨细胞在组织外植体和琼脂糖结构。在软骨细胞构建体中，TGF-β1诱导先前在成熟牛软骨中检测到的120-kDa聚集蛋白聚糖种类的积累。纤维软骨细胞接种的结构含有高分子量的聚集蛋白聚糖，但缺乏聚集蛋白聚糖酶产生的片段中发现的天然的，不成熟的半月板。此外，反映较少的基质积累，纤维软骨细胞结构有显着较低的压缩模量比软骨细胞结构。聚集蛋白聚糖合成、保留和加工的这些细胞类型特异性差异可能对功能性工程组织移植物的开发具有影响。
Aggrecan is an extracellular matrix molecule that contributes to the mechanical properties of articular cartilage and meniscal fibrocartilage, but the abundance and processing of aggrecan in these tissues are different. The objective of this study was to compare patterns of aggrecan processing by chondrocytes and meniscal fibrochondrocytes in tissue explants and cell–agarose constructs. The effects of transforming growth factor-beta 1 (TGF-β1) stimulation on aggrecan deposition and processing were examined, and construct mechanical properties were measured. Fibrochondrocytes synthesized and retained less proteoglycans than did chondrocytes in tissue explants and agarose constructs. In chondrocyte constructs, TGF-β1 induced the accumulation of a 120-kDa aggrecan species previously detected in mature bovine cartilage. Fibrochondrocyte-seeded constructs contained high-molecular-weight aggrecan but lacked aggrecanase-generated fragments found in native, immature meniscus. In addition, reflecting the lesser matrix accumulation, fibrochondrocyte constructs had significantly lower compression moduli than did chondrocyte constructs. These cell type–specific differences in aggrecan synthesis, retention, and processing may have implications for the development of functional engineered tissue grafts.
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