Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction.
Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction.
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DOI：
10.1038/s41368-021-00155-z
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发表时间：
2022-01-25
影响因子：
14.9
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Gong X;Zhang H;Xu X;Ding Y;Yang X;Cheng Z;Tao D;Hu C;Xiang Y;Sun Y
神经嵴源性间充质干细胞（MSCs）在牙齿和骨骼发育过程中发挥重要作用。PRX1+细胞是一种重要的MSC亚型，与成骨有关。然而，它们在牙齿发育和再生中的潜在功能尚不清楚。在本研究中，我们首先评估了小鼠磨牙发育和牙周韧带（PDL）形成过程中PRX1+细胞的命运。通过单细胞RNA测序分析，研究PRX1+细胞在PDL细胞中的分布。采用同种异体移植牙模型研究PRX1+细胞在PDL重建过程中的行为。虽然PRX1+细胞具有空间特异性，可以在第一磨牙分化为几乎所有类型的间充质细胞，但其在第三磨牙的分布非常有限。PDL的形成与大量PRX1+细胞有关；在移植牙PDL重建过程中，来自受体牙槽骨的PRX1+细胞作为周细胞参与血管生成。总的来说，PRX1+细胞是牙MSCs的一个关键亚型，参与小鼠磨牙和PDL的形成，并在牙齿移植后PDL重建过程中作为周细胞参与血管生成。
Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1+ cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1+ cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1+ cells in PDL cells. The behavior of PRX1+ cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1+ cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1+ cells; during transplanted teeth PDL reconstruction, PRX1+ cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1+ cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.
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影响因子：
13.6
作者：
Ortiz A;Bailey SE;Schwartz GT;Hublin JJ;Skinner MM
通讯作者：
Skinner MM
DOI：
10.1002/jbmr.4410
发表时间：
2021-08
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Mizoguchi T;Ono N
通讯作者：
Ono N
影响因子：
168.9
作者：
Seo, BM;Miura, M;Shi, ST
通讯作者：
Shi, ST
影响因子：
7.6
作者：
Kim, T. H.;Bae, C. H.;Cho, E. S.
通讯作者：
Cho, E. S.
C-KIT 表达区分胎儿和产后骨骼祖细胞
影响因子：
5.9
作者：
He, Di Demi;Tang, Xinyu Thomas;Zhou, Bo O.
通讯作者：
Zhou, Bo O.