Experimental study on filtering, transporting, concentrating and focusing of microparticles based on optically induced dielectrophoresis
Experimental study on filtering, transporting, concentrating and focusing of microparticles based on optically induced dielectrophoresis
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基于光诱导介电泳的微粒过滤、输送、浓缩和聚焦实验研究
DOI：
10.1007/s11431-010-4057-6
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发表时间：
2010-08
影响因子：
4.6
通讯作者：
Yin ZhiFeng
中科院分区：
文献类型：
--
作者：
Gao ZhiQiang;Ni ZhongHua;Zhu XiaoLu;Yin ZhiFeng
介电电泳应用的关键问题是提供可动态重构的微电极和低成本的生物粒子操纵方法。基于光电导效应的光致电泳（ODEP）的出现为解决上述问题提供了一种可能。本文设计并制作了ODEP芯片，搭建了相应的实验平台，对基于ODEP的过滤、传输、浓缩和聚焦四种粒子操作方案进行了实验验证，并对操作性能进行了定量分析。实验结果表明，ODEP操纵的功能和性能与光学图形的几何形状、尺度和速度、激励信号频率以及溶液的电导率密切相关。通过增加光学线宽，可以使操作效率提高50%以上。此外，在粒子聚焦操作中，光学斜线的倾斜角度对效率有明显的影响。粒子的最大速度随光阱环内径和厚度的增大而增大。粒子操作效率总是与信号频率和溶液电导率相关，经验上，当溶液电导率在5×10 - 4S/m至5×10 - 3S/m的范围内时，可获得令人满意的性能和高效率。
The key problem to be solved for the dielectrophoresis (DEP) application is to provide dynamically reconfigurable microelectrodes and low-cost methodology for bioparticle manipulation. The emergence of optically induced DEP (ODEP) based on photoconductive effect provides a potential solution for the above problem. In this paper, an ODEP chip is designed and fabricated, and the corresponding experimental platform was established, whereupon four types of particle manipulation regimes-filtering, transporting, concentrating and focusing based on ODEP are experimentally demonstrated and the operating performances are quantitatively analyzed. The experiment results show that the functions and performances of ODEP manipulation are heavily dependent on the geometrical shape, scales and speed of optical patterns, actuating signal frequency and the electric conductivity of the solution. The manipulation efficiency can increase by more than 50% via increasing the optical line width. Moreover, the efficiency is obviously affected by the inclination angle of the optical oblique lines in the manipulation of particle focusing. Additionally, the maximum velocity of particles increases with the increment of the inside radius and the thickness of the optical trapping ring. Particle manipulation efficiency is always related to signal frequency and solution conductivity, and empirically, satisfactory performance and high efficiency are obtained when the solution electric conductivity ranges from 5×10−4S/m to 5×10−3S/m.
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DOI：
10.1109/icma.2009.5246138
发表时间：
2009-09
期刊：
2009 International Conference on Mechatronics and Automation
影响因子：
--
作者：
Xiaolu Zhu;Hong Yi;Z. Ni;Xingzhong Gu
通讯作者：
Xiaolu Zhu;Hong Yi;Z. Ni;Xingzhong Gu
DOI：
10.1109/ias.1995.530460
发表时间：
1995-10
期刊：
IAS '95. Conference Record of the 1995 IEEE Industry Applications Conference Thirtieth IAS Annual Meeting
影响因子：
--
作者：
Xiao-Bo Wang;Ying Huang;P. Gascoyne;Frederick F. Becker
通讯作者：
Xiao-Bo Wang;Ying Huang;P. Gascoyne;Frederick F. Becker
DOI：
10.1016/s1535-5535-04-00234-5
发表时间：
2002-12
期刊：
Journal of Laboratory Automation
影响因子：
--
作者：
F. Schönfeld;A. Griebel;R. Konrad;S. Rink;F. Karlsen
通讯作者：
F. Schönfeld;A. Griebel;R. Konrad;S. Rink;F. Karlsen
影响因子：
2.9
作者：
Gagnon, Z;Chang, HC
通讯作者：
Chang, HC
DOI：
10.1007/s11431-009-0104-6
发表时间：
2009-09
期刊：
Science in China Series E: Technological Sciences
影响因子：
--
作者：
Z. Ni;Hong Yi;Shucun Zhu;Chunfeng Song
通讯作者：
Z. Ni;Hong Yi;Shucun Zhu;Chunfeng Song