In vitro susceptibility of 10 clinical isolates of SARS coronavirus to selected antiviral compounds.
In vitro susceptibility of 10 clinical isolates of SARS coronavirus to selected antiviral compounds.
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DOI：
10.1016/j.jcv.2004.03.003
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发表时间：
2004-09
期刊：
影响因子：
--
通讯作者：
Yuen KY
中科院分区：
文献类型：
--
作者：
Chen F;Chan KH;Jiang Y;Kao RY;Lu HT;Fan KW;Cheng VC;Tsui WH;Hung IF;Lee TS;Guan Y;Peiris JS;Yuen KY
目前迫切需要有效的抗病毒药物，以对抗严重急性呼吸系统综合症（SARS）可能卷土重来。用中和试验和空斑减少试验对10株SARS冠状病毒临床分离株进行了抗病毒药物和中药纯品的筛选。干扰素-β-1a、白细胞干扰素-α、利巴韦林、洛匹那韦、金刚乙胺、黄芩苷和黄芩苷显示出抗病毒活性。这两种干扰素只有在病毒接种前16小时将细胞系与药物预孵育时才有活性。结果通过空斑减少试验证实。抗病毒活性随不同细胞系的使用而变化。进行了协同作用的生物板测定，显示干扰素β-1a或白细胞干扰素-α与利巴韦林的组合具有协同作用。由于这些药物的临床和毒性特征是众所周知的，因此在未来流行病的随机安慰剂对照试验中，应考虑将其单独或联合用于预防或治疗SARS。
Effective antiviral agents are urgently needed to combat the possible return of severe acute respiratory syndrome (SARS). Commercial antiviral agents and pure chemical compounds extracted from traditional Chinese medicinal herbs were screened against 10 clinical isolates of SARS coronavirus by neutralisation tests with confirmation by plaque reduction assays. Interferon-beta-1a, leukocytic interferon-alpha, ribavirin, lopinavir, rimantadine, baicalin and glycyrrhizin showed antiviral activity. The two interferons were only active if the cell lines were pre-incubated with the drugs 16 h before viral inoculation. Results were confirmed by plaque reduction assays. Antiviral activity varied with the use of different cell lines. Checkerboard assays for synergy were performed showing combinations of interferon beta-1a or leukocytic interferon-alpha with ribavirin are synergistic. Since the clinical and toxicity profiles of these agents are well known, they should be considered either singly or in combination for prophylaxis or treatment of SARS in randomised placebo controlled trials in future epidemics.
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影响因子：
6.4
作者：
HAYDEN, FG;GWALTNEY, JM
通讯作者：
GWALTNEY, JM
影响因子：
7.6
作者：
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通讯作者：
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DOI：
10.1006/bbrc.2000.3485
发表时间：
2000-09-24
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
10
作者：
Chu, CM;Cheng, VCC;Yuen, KY
通讯作者：
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影响因子：
7.6
作者：
LEBWOHL, M;SACKS, S;PLASSE, T
通讯作者：
PLASSE, T