Autoantibodies to intracellular proteins in human systemic lupus erythematosus are not due to random polyclonal B cell activation.
Autoantibodies to intracellular proteins in human systemic lupus erythematosus are not due to random polyclonal B cell activation.
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人类系统性红斑狼疮细胞内蛋白的自身抗体并非由随机多克隆 B 细胞激活所致。
DOI：
10.1002/art.1780311101
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发表时间：
1988
影响因子：
--
通讯作者：
Elkon,KB
中科院分区：
文献类型：
--
作者：
Gharavi,AE;Chu,JL;Elkon,KB
比较了从哺乳动物来源(HeLa细胞)和原核来源(大肠杆菌)提取的总蛋白与系统性红斑狼疮(SLE)患者血清和正常人血清的抗体结合情况。当在免疫印迹上比较免疫印迹上免疫球蛋白抗体识别的平均多肽或蛋白质时，系统性红斑狼疮血清与HeLa细胞提取物中蛋白质的结合数量明显多于正常人血清(P<；0.001)。相反，SLE患者的血清与正常对照相比，实际上结合的是结肠蛋白，尽管差异在统计学上不显著。单个SLE血清识别的E蛋白和HeLa蛋白之间无相关性，在高水平的血清中也没有与大量抗原发生反应的趋势。SLE血清中的免疫球蛋白在变性或天然状态下均不与6种纯化的真核蛋白标准品(仅根据大小和电荷的不同而选择)结合。这些发现表明，SLE患者中高水平的抗真核细胞内蛋白的抗体不能用随机的多克隆B细胞激活来解释。
Antibody binding to total protein extracted from a mammalian source (HeLa cells) and from a prokaryotic source (Escherichia coli) was compared in sera from patients with systemic lupus erythematosus (SLE) and sera from normal subjects. When the average numbers of peptides or proteins recognized by IgG antibodies were compared on immunoblots, SLE sera bound to a significantly greater number of proteins from the HeLa cell extract than did sera from normal individuals (P< 0.001). In contrast, SLE sera actually bound to fewerE coliproteins than did the sera obtained from normal controls, although the difference was not statistically significant. There was no correlation between the number ofE coliproteins and HeLa proteins recognized by individual SLE sera, and there was no trend toward reactivity with a larger number of antigens in sera containing higher levels of IgG. IgG from SLE sera did not bind to 6 purified eukaryotic protein standards (selected solely on the basis of differences in size and charge) either in their denatured state or in their native state. These findings indicate that the high levels of IgG antibodies against selected eukaryotic intracellular proteins in patients with SLE cannot be explained by a random polyclonal B cell activation.
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