Functionalized PEG hydrogels through reactive dip-coating for the formation of immunoactive barriers.
Functionalized PEG hydrogels through reactive dip-coating for the formation of immunoactive barriers.
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DOI：
10.1016/j.biomaterials.2011.04.049
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发表时间：
2011-09
期刊：
影响因子：
14
通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
--
作者：
Hume, Patrick S.;Bowman, Christopher N.;Anseth, Kristi S.
通过植入式细胞递送装置影响宿主免疫系统有可能减少移植部位的炎症并增加组织接受的可能性。为了实现这一目标，酶促引发的浸涂技术适于制造保形水凝胶层，并在载有细胞的聚（乙二醇）（PEG）水凝胶上产生免疫活性聚合物涂层。葡萄糖氧化酶（GOx）引发的浸涂能够在PEG水凝胶表面快速形成均匀的PEG基涂层，厚度可达500 μm，厚度与反应时间成正比。通过硫醇化生物分子，随后通过硫醇-丙烯酸酯共聚共价结合到涂层中来制造生物功能涂层。通过荧光共聚焦显微镜和改良的ELISA验证这些蛋白质的存在，其表明可实现高达13±1 ng /包被cm 2的IgG浓度。将已知诱导T细胞凋亡的抗Fas抗体掺入涂层中，加入或不加入ICAM-1以促进T细胞与功能化涂层的相互作用。将Jurkat T细胞接种在功能化涂层上，并测量细胞凋亡的诱导作为涂层生物活性的指标。在与功能化涂层相互作用48小时后，所有细胞的61±9%凋亡或死亡，而非功能化涂层上的T细胞仅为18±5%。最后，通过在含有PEG甲基丙烯酸酯涂层的凝胶内用包封的β-细胞或3 T3成纤维细胞修饰凝胶来证实表面引发的GOx涂层过程的细胞相容性。
Influencing the host immune system via implantable cell-delivery devices has the potential to reduce inflammation at the transplant site and increase the likelihood of tissue acceptance. Towards this goal, an enzymatically-initiated, dip-coating technique is adapted to fabricate conformal hydrogel layers and to create immunoactive polymer coatings on cell-laden poly(ethylene glycol) (PEG) hydrogels. Glucose oxidase (GOx)-initiated dip coatings enable the rapid formation of uniform, PEG-based coatings on the surfaces of PEG hydrogels, with thicknesses up to 500 μm where the thickness is proportional to the reaction time. Biofunctional coatings were fabricated by thiolating biomolecules that were subsequently covalently incorporated into the coating layer via thiol-acrylate copolymerization. The presence of these proteins was verified via fluorescent confocal microscopy and a modified ELISA, which indicated IgG concentrations as high as 13±1 ng / coated cm2 were achievable. Anti-Fas antibody, known to induce T cell apoptosis, was incorporated into coatings, with or without the addition of ICAM-1 to promote T cell interaction with the functionalized coating. Jurkat T cells were seeded atop functionalized coatings and the induction of apoptosis was measured as an indicator of coating bioactivity. After 48 hours of interaction with the functionalized coatings, 61±9% of all cells were either apoptotic or dead, compared to only 18±5% of T cells on non-functionalized coatings. Finally, the cytocompatibility of the surface-initiated GOx coating process was confirmed by modifying gels with either encapsulated β-cells or 3T3 fibroblasts within a gel that contained a PEG methacrylate coating.
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DOI：
10.1084/jem.180.4.1547
发表时间：
1994-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cifone MG;De Maria R;Roncaioli P;Rippo MR;Azuma M;Lanier LL;Santoni A;Testi R
通讯作者：
Testi R
影响因子：
14
作者：
Jang, JY;Lee, DY;Byun, Y
通讯作者：
Byun, Y
影响因子：
6.2
作者：
Lin, Chien-Chi;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
19
作者：
Cheung, Charles Y.;McCartney, Suzanne J.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
3.3
作者：
Cruise, GM;Hegre, OD;Hubbell, JA
通讯作者：
Hubbell, JA