The effects of denatured major bovine whey proteins on the digestive tract, assessed by Caco-2 cell differentiation and on viability of suckling mice
The effects of denatured major bovine whey proteins on the digestive tract, assessed by Caco-2 cell differentiation and on viability of suckling mice
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通过 Caco-2 细胞分化评估变性主要牛乳清蛋白对消化道的影响以及对乳鼠活力的影响
DOI：
10.1017/s0022029921000376
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发表时间：
2021
影响因子：
2.1
通讯作者：
Yoshihiro Kanamaru
中科院分区：
文献类型：
--
作者：
Chihiro Kobayashi;Mizuho Inagaki;Midori Nohara;Mayuko Fukuoka;Xijier;Tomio Yabe;Yoshihiro Kanamaru
牛乳乳清中含有α-乳白蛋白（α-LA）和β-乳球蛋白（β-LG）。已知化学和物理处理可以改变这些蛋白质的构象，我们以前曾报道过用三氟乙醇（TFE）变性并从灭菌的市售牛奶中分离的α-LA抑制大鼠隐窝IEC-6细胞的生长。在本研究中，我们旨在使用培养的肠细胞评价TFE处理的α-LA和β-LG对细胞生长的影响，并使用乳鼠模型评价其安全性。首先，我们研究了TFE处理的乳清蛋白对不同分化阶段的人结肠Caco-2细胞的影响。在未分化阶段，我们通过水溶性四唑-1方法评估细胞生长。天然乳清蛋白增强细胞增殖，而TFE处理的乳清蛋白强烈抑制细胞生长。我们通过测量跨上皮电阻（TER）来研究分化后阶段的细胞屏障功能。不仅天然的，而且TFE处理的乳清蛋白增加TER。接下来，我们评估了TFE处理的α-LA和β-LG是否对健康乳鼠有不良影响。给予TFE处理的样品的小鼠没有显示任何不良症状。我们还使用人轮状病毒感染的胃肠道疾病乳鼠模型进行了安全性试验。即使是TFE处理的乳清蛋白似乎也能防止腹泻症状的发展，而没有任何副作用。虽然我们无法知道长期摄入变性乳清蛋白的影响，但这些结果表明，它们对分化的肠细胞和消化道没有不良影响，至少在短期摄入中是如此。
Alpha-lactalbumin (α-LA) and β-lactoglobulin (β-LG) are contained in bovine milk whey. Chemical and physical treatments are known to alter the conformation of these proteins and we have previously reported that α-LA denatured with trifluoroethanol (TFE) and isolated from sterilized market milk inhibited the growth of rat crypt IEC-6 cells. In the present study, we aimed to evaluate the effects of TFE-treated α-LA and β-LG on cell growth using cultured intestinal cells and on their safety using a suckling mouse model. First, we investigated the effect of the TFE-treated whey proteins on human colonic Caco-2 cells at various differentiation stages. In the undifferentiated stage, we assessed cell growth by a water-soluble tetrazolium-1 method. The native whey proteins enhanced cell proliferation, whereas the TFE-treated whey proteins strongly inhibited cell growth. We investigated cell barrier function in the post-differentiated stage by measuring transepithelial electrical resistance (TER). Not only native but also the TFE-treated whey proteins increased TER. Next, we evaluated whether the TFE-treated α-LA and β-LG have adverse effects on healthy suckling mice. No mice given by the TFE-treated samples showed any adverse symptoms. We also performed a safety test using a human rotavirus infected gastrointestinal disease suckling mice model. Even the TFE-treated whey proteins appeared to prevent the development of diarrheal symptoms without any adverse effects. Although we cannot know the effect of long-term ingestion of denatured whey proteins, these results suggest that they have no adverse effects on differentiated intestinal cells and digestive tract, at least in short-term ingestion.
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DOI：
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期刊：
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影响因子：
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DOI：
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发表时间：
1998
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DOI：
10.1016/j.ijbiomac.2015.05.045
发表时间：
2015
影响因子：
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作者：
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DOI：
10.1271/bbb.59.1951
发表时间：
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期刊：
Bioscience, biotechnology, and biochemistry
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