Supplemental dietary whey protein concentrate reduces rotavirus-induced disease symptoms in suckling mice.
Supplemental dietary whey protein concentrate reduces rotavirus-induced disease symptoms in suckling mice.
复制标题
补充膳食乳清蛋白浓缩物可减少乳鼠轮状病毒引起的疾病症状。
DOI：
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复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
D. Dey
中科院分区：
文献类型：
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作者：
F. Wolber;Anne M Broomfield;L. Fray;M. L. Cross;D. Dey
轮状病毒引起的腹泻是一种常见的感染，每年导致近50万儿童死亡。目前，尚不存在大规模的预防性治疗或疫苗。由于一些乳清蛋白浓缩物(WPC)被证明含有生物活性成分，可以激活免疫细胞和/或预防感染，本研究旨在评估专有WPC IMUCARE(WPC-IC)是否可以预防轮状病毒。用WPC-IC或对照蛋白牛血清白蛋白灌胃给9~17d的BALB/c乳鼠，于11d龄时感染小鼠轮状病毒，疾病症状分为轻、中、重三级，感染后取粪便标本检测病毒脱落。63%的对照组小鼠出现严重腹泻；而喂食WPC-IC的小鼠，这一比例显著降低至36%。对照组出现4d的严重腹泻，而WPC-IC组只有2d的严重腹泻。虽然每只小鼠的平均病毒载量在两组之间没有差异，但WPC-IC组小鼠在感染后第13、16和17天从粪便中排出高水平病毒的比例显著低于对照组，在第14天显著高于对照组。血清和肠液中轮状病毒特异性抗体水平在两组之间没有差异。因此，使用WPC-IC进行预防性治疗可以通过降低严重腹泻的患病率和缩短出现严重症状和病毒大量脱落的时间来减少轮状病毒病。
Rotavirus-induced diarrhea is a common infection that results in the death of nearly 500,000 children annually. Currently, no large-scale preventative treatments or vaccines exist. Because some whey protein concentrates (WPC) were shown to contain bioactive ingredients that may activate immune cells and/or prevent infection, the current study was conducted to assess whether the proprietary WPC IMUCARE (WPC-IC) could protect against rotavirus. Suckling BALB/c mice were treated by gavage once daily with WPC-IC or with the control protein bovine serum albumin from the age of 9 to 17 d, and were infected with murine rotavirus at the age of 11 d. Disease symptoms were graded as mild, moderate, or severe, and viral shedding was measured in fecal samples during the postinfection period. Severe diarrhea occurred in 63% of control mice; this was significantly reduced to 36% in WPC-IC-fed mice. Severe diarrhea occurred for a 4-d period in the control group but only for a 2-d period in the WPC-IC group. Although the mean viral load per mouse did not differ between the groups, the proportion of mice shedding high levels of the virus in the feces postinfection was significantly lower in the WPC-IC group on d 13, 16, and 17, and significantly higher on d 14. Rotavirus-specific antibody levels in serum and gut fluid did not differ between groups. Thus, prophylactic treatment with WPC-IC may reduce rotaviral disease by decreasing the prevalence of severe diarrhea and by decreasing the time period during which severe symptoms and high viral shedding occur.
肠道粘蛋白以寡糖依赖性方式抑制轮状病毒复制。
DOI：
10.1093/infdis/169.5.1002
发表时间：
1994
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Yolken,RH;Ojeh,C;Khatri,IA;Sajjan,U;Forstner,JF
通讯作者：
Forstner,JF
影响因子：
29.4
作者：
CHEN, CC;BAYLOR, M;BASS, DM
通讯作者：
BASS, DM
上皮细胞对轮状病毒感染的反应。
DOI：
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发表时间：
1999
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Rollo,EE;Kumar,KP;Reich,NC;Cohen,J;Angel,J;Greenberg,HB;Sheth,R;Anderson,J;Oh,B;Hempson,SJ;Mackow,ER;Shaw,RD
通讯作者：
Shaw,RD