Fighting against fast speckle decorrelation for light focusing inside live tissue by photon frequency shifting.
Fighting against fast speckle decorrelation for light focusing inside live tissue by photon frequency shifting.
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DOI：
10.1021/acsphotonics.0c00027
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发表时间：
2020-03-18
期刊：
影响因子：
7
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Yang J;Li L;Li J;Cheng Z;Liu Y;Wang LV
利用数字光学相位共轭（DOPC）技术将光聚焦到活体组织内，在深部组织成像、光遗传学、显微外科和光疗等领域具有广泛的应用前景。然而，活体动物中的快速生理运动（诸如血流和呼吸运动）产生不期望的光子扰动，并且因此不可避免地劣化光聚焦的性能。在这里，我们开发了一种光子频移DOPC方法，通过以独特的频率切换引导星星的状态来对抗快速的生理运动。因此，被引导星星标记的光子在特定频率下被很好地检测到，将它们与被快速运动扰动的光子分开。光聚焦被证明在体外的幻影和小鼠在体内与实质上改善聚焦对比度。这项工作为活组织内的光聚焦提供了新的视角，并有望在生物医学领域得到广泛的应用。
Focusing light inside live tissue by digital optical phase conjugation (DOPC) has been intensively investigated due to its potential biomedical applications in deep-tissue imaging, optogenetics, microsurgery, and phototherapy. However, fast physiological motions in a live animal, such as blood flow and respiratory motions, produce undesired photon perturbation and thus inevitably deteriorate the performance of light focusing. Here, we develop a photon-frequency-shifting DOPC method to fight against fast physiological motions by switching the states of a guide star at a distinctive frequency. Therefore, the photons tagged by the guide star are well detected at the specific frequency, separating them from the photons perturbed by fast motions. Light focusing was demonstrated in both phantoms in vitro and mice in vivo with substantially improved focusing contrast. This work puts a new perspective on light focusing inside live tissue and promises wide biomedical applications.
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影响因子：
3.6
作者：
Liu Y;Ma C;Shen Y;Wang LV
通讯作者：
Wang LV
数字光相结合，用于通过浑浊介质传递二维图像。
影响因子：
4.6
作者：
Hillman, Timothy R.;Yamauchi, Toyohiko;Choi, Wonshik;Dasari, Ramachandra R.;Feld, Michael S.;Park, YongKeun;Yaqoob, Zahid
通讯作者：
Yaqoob, Zahid
影响因子：
35
作者：
通讯作者：
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影响因子：
10.4
作者：
Ma C;Zhou F;Liu Y;Wang LV
通讯作者：
Wang LV
影响因子：
10.4
作者：
Ruan H;Haber T;Liu Y;Brake J;Kim J;Berlin JM;Yang C
通讯作者：
Yang C