A highly accurate inclusive cancer screening test using Caenorhabditis elegans scent detection.
A highly accurate inclusive cancer screening test using Caenorhabditis elegans scent detection.
复制标题
使用秀丽隐杆线虫味检测的高度准确的纳入癌症筛查测试。
DOI：
10.1371/journal.pone.0118699
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hamakawa M
中科院分区：
文献类型：
--
作者：
Hirotsu T;Sonoda H;Uozumi T;Shinden Y;Mimori K;Maehara Y;Ueda N;Hamakawa M
早期发现和治疗对各种癌症的成功根除至关重要，而开发经济的、非侵入性的新型癌症筛查系统至关重要。以前使用犬类气味检测的报告证明了癌症特异性气味的存在。然而，由于需要保持准确性，将犬类气味识别引入临床实践是困难的。在这项研究中，我们开发了一种线虫气味检测试验(NSDT)，以提供一种经济、无痛、快速和方便的新型高精度癌症检测系统。我们发现，野生型线虫对人类癌细胞分泌物、癌症组织和癌症患者的尿液表现出诱人的趋化性，但避免了对照尿液；同时，线虫嗅觉神经元对癌症患者尿液的反应明显强于对照尿液。相比之下，G蛋白α突变体和嗅觉神经元切除的动物不会被癌症患者的尿液吸引，这表明线虫能感觉到尿液中的气味。我们测试了242个样本来测量NSDT的性能，发现其敏感度为95.8%；这明显高于其他现有的肿瘤标志物。特异度为95.0%。重要的是，这项测试能够诊断早期(0期或1期)测试的各种癌症类型。总之，基于线虫气味的分析可能提供一种新的策略来检测和研究与疾病相关的气味。
Early detection and treatment are of vital importance to the successful eradication of various cancers, and development of economical and non-invasive novel cancer screening systems is critical. Previous reports using canine scent detection demonstrated the existence of cancer-specific odours. However, it is difficult to introduce canine scent recognition into clinical practice because of the need to maintain accuracy. In this study, we developed a Nematode Scent Detection Test (NSDT) using Caenorhabditis elegans to provide a novel highly accurate cancer detection system that is economical, painless, rapid and convenient. We demonstrated wild-type C. elegans displayed attractive chemotaxis towards human cancer cell secretions, cancer tissues and urine from cancer patients but avoided control urine; in parallel, the response of the olfactory neurons of C. elegans to the urine from cancer patients was significantly stronger than to control urine. In contrast, G protein α mutants and olfactory neurons-ablated animals were not attracted to cancer patient urine, suggesting that C. elegans senses odours in urine. We tested 242 samples to measure the performance of the NSDT, and found the sensitivity was 95.8%; this is markedly higher than that of other existing tumour markers. Furthermore, the specificity was 95.0%. Importantly, this test was able to diagnose various cancer types tested at the early stage (stage 0 or 1). To conclude, C. elegans scent-based analyses might provide a new strategy to detect and study disease-associated scents.
登录
查看更多内容
影响因子：
2
作者：
Nuttley, WM;Harbinder, S;van der Kooy, D
通讯作者：
van der Kooy, D
影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
12.6
作者：
Di Natale, C;Macagnano, A;D'Amico, A
通讯作者：
D'Amico, A
影响因子：
168.9
作者：
Phillips, M;Gleeson, K;McVay, WP
通讯作者：
McVay, WP
DOI：
10.1517/17530059.2012.665870
发表时间：
2012-05-01
期刊：
Expert opinion on medical diagnostics
影响因子：
--
作者：
D'Amico, Arnaldo;Di Natale, Corrado;Sterk, Peter Jason
通讯作者：
Sterk, Peter Jason