Bacterial community mapping of the mouse gastrointestinal tract.
Bacterial community mapping of the mouse gastrointestinal tract.
复制标题
小鼠胃肠道的细菌群落图。
DOI：
10.1371/journal.pone.0074957
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wu XL
中科院分区：
文献类型：
--
作者：
Gu S;Chen D;Zhang JN;Lv X;Wang K;Duan LP;Nie Y;Wu XL
维持哺乳动物胃肠道（GI）群落的平衡对宿主健康维护至关重要。然而，我们对胃肠道微生物群落的了解仍然非常有限。在这项研究中，从C57 BL/6小鼠胃肠道采集的样品进行了基于16 S rRNA基因序列的分析，以检查小鼠胃肠道沿着的特征性细菌群落，包括存在于胃、十二指肠、空肠、回肠、盲肠、结肠和粪便中的细菌群落。对283，234个有效序列的进一步分析表明，胃、十二指肠、大肠和粪便样品的系统发育多样性高于空肠和回肠样品。在小肠和胃中发现的微生物群落与大肠和粪便样本中观察到的不同。在胃和小肠中发现了较大比例的乳杆菌科，而在大肠和粪便中发现了较大比例的厌氧菌，如拟杆菌科、普雷沃氏菌科、Rikenellaceae、毛螺菌科和瘤胃球菌科。此外，在大肠和粪便样品之间观察到微生物群的小鼠间差异，其远小于胃和小肠样品之间的差异。据我们所知，我们的研究是第一个系统地表征C57 BL/6小鼠胃肠道细菌群落的研究。
Keeping mammalian gastrointestinal (GI) tract communities in balance is crucial for host health maintenance. However, our understanding of microbial communities in the GI tract is still very limited. In this study, samples taken from the GI tracts of C57BL/6 mice were subjected to 16S rRNA gene sequence-based analysis to examine the characteristic bacterial communities along the mouse GI tract, including those present in the stomach, duodenum, jejunum, ileum, cecum, colon and feces. Further analyses of the 283,234 valid sequences obtained from pyrosequencing revealed that the gastric, duodenal, large intestinal and fecal samples had higher phylogenetic diversity than the jejunum and ileum samples did. The microbial communities found in the small intestine and stomach were different from those seen in the large intestine and fecal samples. A greater proportion of Lactobacillaceae were found in the stomach and small intestine, while a larger proportion of anaerobes such as Bacteroidaceae, Prevotellaceae, Rikenellaceae, Lachnospiraceae, and Ruminococcaceae were found in the large intestine and feces. In addition, inter-mouse variations of microbiota were observed between the large intestinal and fecal samples, which were much smaller than those between the gastric and small intestinal samples. As far as we can ascertain, ours is the first study to systematically characterize bacterial communities from the GI tracts of C57BL/6 mice.
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影响因子：
17.1
作者：
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