Selection strategies for the development of maize introgression populations.
Selection strategies for the development of maize introgression populations.
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DOI：
10.1371/journal.pone.0092429
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Frisch M
中科院分区：
文献类型：
--
作者：
Herzog E;Falke KC;Presterl T;Scheuermann D;Ouzunova M;Frisch M
渗入库是QTL检测和育种的宝贵资源，但其开发成本高且耗时长。有限个体数量的渐渗群体发展的选择策略和高通量（HT）标记分析是必需的。我们的模拟研究的目的是设计和比较不同DH和杂交方案下100个种群规模为360-720个/代的玉米渐渗群体的选择策略。供体染色体完整或供体染色体一半的预选择减少了同时回交程序的数量。所研究的杂交和选择方案在创建具有明确分离、均匀分布的目标供体染色体片段的渐渗群体方面存在很大差异。DH杂交方案优于交叉方案，主要是由于完全纯合性，这大大减少了渐渗群体中不相交的基因组片段的总数。该杂交方案在选择方面更加灵活，并提供了比DH杂交方案更经济的选择。对于DH杂交方案，在回交世代中逐渐增加种群规模是有利的，因为与恒定种群规模相比，它减少了所需的HT测定总数。对于杂交方案，大群体规模的最终回交代有利于最终回交代目标片段的选择，减少了大供体染色体片段的固定。所提出的杂交和选择方案有助于利用外来种质的遗传多样性，提高作物窄基育种群体的遗传变异。
Introgression libraries are valuable resources for QTL detection and breeding, but their development is costly and time-consuming. Selection strategies for the development of introgression populations with a limited number of individuals and high-throughput (HT) marker assays are required. The objectives of our simulation study were to design and compare selection strategies for the development of maize introgression populations of 100 lines with population sizes of 360–720 individuals per generation for different DH and crossing schemes. Pre-selection for complete donor chromosomes or donor chromosome halves reduced the number of simultaneous backcross programs. The investigated crossing and selection schemes differed considerably with respect to their suitability to create introgression populations with clearly separated, evenly distributed target donor chromosome segments. DH crossing schemes were superior to crossing schemes, mainly due to complete homozygosity, which greatly reduced the total number of disjunct genome segments in the introgression populations. The crossing schemes were more flexible with respect to selection and provided economic alternatives to DH crossing schemes. For the DH crossing schemes, increasing population sizes gradually over backcross generations was advantageous as it reduced the total number of required HT assays compared to constant population sizes. For the crossing schemes, large population sizes in the final backcross generation facilitated selection for the target segments in the final backcross generation and reduced fixation of large donor chromosome segments. The suggested crossing and selection schemes can help to make the genetic diversity of exotic germplasm available for enhancing the genetic variation of narrow-based breeding populations of crops.
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影响因子：
3.1
作者：
Syed, NH;Pooni, HS;Kearsey, MJ
通讯作者：
Kearsey, MJ
影响因子：
9.8
作者：
McCouch S
通讯作者：
McCouch S
影响因子：
4.2
作者：
Mano, Y.;Omori, F.
通讯作者：
Omori, F.
影响因子：
5.4
作者：
Szalma, S. J.;Hostert, B. M.;Holland, J. B.
通讯作者：
Holland, J. B.
影响因子：
5.4
作者：
Falke, K. C.;Susic, Z.;Geiger, H. H.
通讯作者：
Geiger, H. H.