Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation.
Purkinje Cell Activity Determines the Timing of Sensory-Evoked Motor Initiation.
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浦肯野细胞活性决定感觉诱发运动启动的时间。
DOI：
10.1016/j.celrep.2020.108537
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发表时间：
2020-12-22
期刊：
影响因子：
8.8
通讯作者：
Häusser M
中科院分区：
文献类型：
--
作者：
Tsutsumi S;Chadney O;Yiu TL;Bäumler E;Faraggiana L;Beau M;Häusser M
小脑神经元可以发出感觉和运动事件的信号，但它们在主动感觉运动处理中的作用仍不清楚。我们记录和操纵浦肯野细胞的活动在一项任务，要求小鼠迅速区分多感觉和unisensory刺激前电机启动。神经像素记录表明，感觉刺激和运动启动都是由短潜伏期的简单尖峰信号表示的。短潜伏期简单尖峰的光遗传学操纵以速率依赖的方式废除或延迟运动启动，表明在运动启动及其时机中的作用。双光子钙成像揭示了组织成保守的交替旁棘条纹的复杂尖峰的任务相关的一致性。感觉诱发的复合尖峰的连贯性随着学习而增加，并与运动启动的时间精度增强相关。这些结果表明，简单的尖峰和复杂的尖峰支配感觉驱动的运动启动：简单的尖峰调制其潜伏期，复杂的尖峰完善其时间精度，小脑感觉运动控制提供特定的细胞基质。一个多感觉关联任务使小脑参与驱动定时行为简单的尖峰决定是否以及何时启动感觉诱发的运动动作复杂的尖峰信号显示出高度保守的交替旁组织获得的复杂尖峰相干性有助于精确定时的运动启动Tsutsumi et al.联合收割机结合神经像素记录，光遗传学和双光子成像的浦肯野细胞在小脑外侧的多感觉关联任务，揭示了短潜伏期简单的尖峰确定的启动感觉诱发的行动和它的时间，而空间组织和收购的复杂尖峰信号的一致性有助于其时间精度。
Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neuropixels recordings show that both sensory stimuli and motor initiation are represented by short-latency simple spikes. Optogenetic manipulation of short-latency simple spikes abolishes or delays motor initiation in a rate-dependent manner, indicating a role in motor initiation and its timing. Two-photon calcium imaging reveals task-related coherence of complex spikes organized into conserved alternating parasagittal stripes. The coherence of sensory-evoked complex spikes increases with learning and correlates with enhanced temporal precision of motor initiation. These results suggest that both simple spikes and complex spikes govern sensory-driven motor initiation: simple spikes modulate its latency, and complex spikes refine its temporal precision, providing specific cellular substrates for cerebellar sensorimotor control. A multisensory association task engages the cerebellum to drive timed behavior Simple spikes determine whether and when to initiate sensory-evoked motor actions Complex spike signals show highly conserved alternating parasagittal organization Acquired complex spike coherence contributes to precisely timed motor initiation Tsutsumi et al. combine Neuropixels recordings, optogenetics, and two-photon imaging of Purkinje cells in the lateral cerebellum in a multisensory association task to reveal that short-latency simple spikes determine the initiation of sensory-evoked actions and its timing, whereas spatially organized and acquired coherence in complex spike signals contribute to its temporal precision.
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影响因子：
25
作者：
Heffley W;Song EY;Xu Z;Taylor BN;Hughes MA;McKinney A;Joshua M;Hull C
通讯作者：
Hull C
影响因子：
64.8
作者：
Herzfeld DJ;Kojima Y;Soetedjo R;Shadmehr R
通讯作者：
Shadmehr R
影响因子：
7.7
作者：
Heffley, William;Hull, Court
通讯作者：
Hull, Court
影响因子：
16.2
作者：
Chabrol, Francois P.;Blot, Antonin;Mrsic-Flogel, Thomas D.
通讯作者：
Mrsic-Flogel, Thomas D.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y