Filamentous Phytophthora Pathogens Deploy Effectors to Interfere With Bacterial Growth and Motility.
Filamentous Phytophthora Pathogens Deploy Effectors to Interfere With Bacterial Growth and Motility.
复制标题
DOI：
10.3389/fmicb.2020.581511
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Dou D
中科院分区：
文献类型：
--
作者：
Wang J;Shen D;Ge C;Du Y;Lin L;Liu J;Bai T;Jing M;Qian G;Dou D
疫霉由一组丝状植物病原体组成，可在世界范围内引起严重的作物病害。众所周知，疫霉能分泌一系列复杂的效应物质来调节植物免疫，决定抗性和敏感性。人们还认识到，疫霉病原体可能生活在包括细菌在内的复杂环境微生物中的自然生态位中。然而，疫霉病原体如何与其共生的微生物相互作用仍知之甚少。在这里，我们提出了这样一个耐人寻味的案例，通过使用疫霉-细菌相互作用作为一个工作系统。我们发现，在共培养的实验室条件下，几种疫霉病原菌似乎阻止了生态相关细菌的接触，包括荧光假单胞菌和模式菌大肠杆菌。我们进一步观察到，大豆疫霉利用保守的Crinkler(CRN)效应蛋白PsCRN63来抑制细菌的生长。辣椒疫霉部署另一个CRN效应器PcCRN173来干扰细菌鞭毛和/或IV型菌毛介导的运动，而辣椒疫霉衍生的RxLR效应器PcAvh540抑制细菌的游泳运动，但不抑制抽动运动和生物膜的形成，这表明效应器介导的疫霉-细菌相互作用的功能多样化。因此，我们的研究提供了第一个案例，表明丝状疫霉病原菌可以部署效应器来干扰细菌的生长和运动，揭示了疫霉病原菌和细菌物种之间前所未有的效应器介导的跨王国相互作用，从而揭示了丝状植物病原菌中效应器蛋白的生态意义，以及它们在病原菌-植物相互作用中的典型作用。
Phytophthora comprises a group of filamentous plant pathogens that cause serious crop diseases worldwide. It is widely known that a complex effector repertoire was secreted by Phytophthora pathogens to manipulate plant immunity and determine resistance and susceptibility. It is also recognized that Phytophthora pathogens may inhabit natural niches within complex environmental microbes, including bacteria. However, how Phytophthora pathogens interact with their cohabited microbes remains poorly understood. Here, we present such an intriguing case by using Phytophthora–bacteria interaction as a working system. We found that under co-culture laboratory conditions, several Phytophthora pathogens appeared to block the contact of an ecologically relevant bacterium, including Pseudomonas fluorescence and a model bacterium, Escherichia coli. We further observed that Phytophthora sojae utilizes a conserved Crinkler (CRN) effector protein, PsCRN63, to impair bacterial growth. Phytophthora capsici deploys another CRN effector, PcCRN173, to interfere with bacterial flagellum- and/or type IV pilus-mediated motility whereas a P. capsici-derived RxLR effector, PcAvh540, inhibits bacterial swimming motility, but not twitching motility and biofilm formation, suggesting functional diversification of effector-mediated Phytophthora–bacteria interactions. Thus, our studies provide a first case showing that the filamentous Phytophthora pathogens could deploy effectors to interfere with bacterial growth and motility, revealing an unprecedented effector-mediated inter-kingdom interaction between Phytophthora pathogens and bacterial species and thereby uncovering ecological significance of effector proteins in filamentous plant pathogens besides their canonical roles involving pathogen–plant interaction.
登录
查看更多内容
DOI：
10.1073/pnas.1808585115
发表时间：
2018-08-14
影响因子：
11.1
作者：
He Q;Naqvi S;McLellan H;Boevink PC;Champouret N;Hein I;Birch PRJ
通讯作者：
Birch PRJ
DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chang YW;Rettberg LA;Treuner-Lange A;Iwasa J;Søgaard-Andersen L;Jensen GJ
通讯作者：
Jensen GJ
影响因子：
30.3
作者：
Ma LS;Hachani A;Lin JS;Filloux A;Lai EM
通讯作者：
Lai EM
影响因子：
4.8
作者：
Ryjenkov, Dmitri A.;Simm, Roger;Gomelsky, Mark
通讯作者：
Gomelsky, Mark
影响因子：
5
作者：
Han, Yong;Wang, Yan;Tombosa, Simon;Wright, Stephen;Huffman, Justin;Yuen, Gary;Qian, Guoliang;Liu, Fengquan;Shen, Yuemao;Du, Liangcheng
通讯作者：
Du, Liangcheng