Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions.
Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions.
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DOI：
10.1016/j.cell.2013.01.042
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发表时间：
2013-02-14
期刊：
影响因子：
64.5
通讯作者：
Mekalanos JJ
中科院分区：
文献类型：
--
作者：
Basler M;Ho BT;Mekalanos JJ
细菌VI型分泌系统（T6 SS）是细菌用于靶向猎物细胞以通过效应蛋白易位进行抑制的动态细胞器。延时荧光显微镜已经记录了铜绿假单胞菌相邻姐妹细胞中相对的T6 SS细胞器的惊人动态。这种细胞-细胞相互作用被称为“T6 SS决斗”，可能反映了由T6 SS抗菌攻击驱动的生物过程。在这里，我们表明，T6 SS决斗行为强烈影响铜绿假单胞菌捕食异源细菌物种的能力。我们表明，在铜绿假单胞菌的情况下，T6 SS依赖的杀死霍乱弧菌或不动杆菌baylyi是大大刺激T6 SS活性发生在这些猎物物种。我们的数据表明，在铜绿假单胞菌中，T6 SS细胞器组装和致命反击由对应于由第二侵略性T6 SS+细菌细胞阐述的T6 SS装置的攻击点的信号调节。
The bacterial Type VI secretion system (T6SS) is dynamic organelle that bacteria use to target prey cells for inhibition via translocation of effector proteins. Time-lapse fluorescence microscopy has documented striking dynamics of opposed T6SS organelles in adjacent sister cells of Pseudomonas aeruginosa. Such cell-cell interactions have been termed "T6SS dueling" and likely reflect a biological process that is driven by T6SS antibacterial attack. Here we show that T6SS dueling behavior strongly influences the ability of P. aeruginosa to prey upon heterologous bacterial species. We show that in the case of P. aeruginosa, T6SS-dependent killing of either Vibrio cholerae or Acinetobacter baylyi is greatly stimulated by T6SS activity occurring in those prey species. Our data suggest that in P. aeruginosa, T6SS organelle assembly and lethal counterattack are regulated by a signal that corresponds to the point of attack of the T6SS apparatus elaborated by a second aggressive T6SS+ bacterial cell.
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影响因子：
9.9
作者：
de Berardinis, Veronique;Vallenet, David;Castelli, Vanina;Besnard, Marielle;Pinet, Agnes;Cruaud, Corinne;Samair, Sumitta;Lechaplais, Christophe;Gyapay, Gabor;Richez, Celine;Durot, Maxime;Kreimeyer, Annett;Le Fevre, Francois;Schaechter, Vincent;Pezo, Valerie;Doering, Volker;Scarpelli, Claude;Medigue, Claudine;Cohen, Georges N.;Marliere, Philippe;Salanoubat, Marcel;Weissenbach, Jean
通讯作者：
Weissenbach, Jean
影响因子：
64.5
作者：
Kohanski, Michael A.;Dwyer, Daniel J.;Collins, James J.
通讯作者：
Collins, James J.
影响因子：
3.2
作者：
Aoki, S. K.;Webb, J. S.;Low, D. A.
通讯作者：
Low, D. A.
影响因子：
14.9
作者：
Metzgar, D;Bacher, JM;de Crécy-Lagard, V
通讯作者：
de Crécy-Lagard, V
影响因子：
3.6
作者：
Aoki, Stephanie K.;Malinverni, Juliana C.;Jacoby, Kyle;Thomas, Benjamin;Pamma, Rupinderjit;Trinh, Brooke N.;Remers, Susan;Webb, Julia;Braaten, Bruce A.;Silhavy, Thomas J.;Low, David A.
通讯作者：
Low, David A.