Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB.
Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB.
复制标题
DOI：
10.1111/j.1365-2958.2008.06404.x
复制
发表时间：
2008-10
影响因子：
3.6
通讯作者：
Low, David A.
中科院分区：
文献类型：
--
作者：
Aoki, Stephanie K.;Malinverni, Juliana C.;Jacoby, Kyle;Thomas, Benjamin;Pamma, Rupinderjit;Trinh, Brooke N.;Remers, Susan;Webb, Julia;Braaten, Bruce A.;Silhavy, Thomas J.;Low, David A.
接触依赖性生长抑制（CDI）是一种现象，细菌细胞生长通过CdiA/CdiB双组分分泌系统经细胞间直接接触来调控。对CDI抗性突变体的特性研究使我们能够确定BamA（YaeT）是CDI的外膜受体，AcrB是一个潜在的下游靶点。值得注意的是，BamA和AcrB都是不同多组分机器的一部分。Bam机器将外膜β-桶状蛋白（OMPs）组装到外膜中，Acr机器将小分子输出到细胞外环境。我们发现，一个降低BamA表达的突变会减少CDI⁺抑制细胞的结合，这通过用荧光标记细菌进行流式细胞术测量。此外，基于免疫荧光识别BamA细胞外表位的α -BamA抗体，特异性地阻断了抑制细胞 - 靶细胞的结合以及CDI。鉴定出的第二类CDI抗性突变体在acrB基因中携带无效突变。AcrB是一个多药外排泵的内膜组分，它通常与膜融合蛋白AcrA和外膜蛋白TolC形成一个跨细胞包膜的复合物。引人注目的是，CDI对BamA和AcrB蛋白的需求与其多组分机器无关，因此它们在CDI途径中的作用可能反映了新的、与输入相关的功能。
Contact-dependent growth inhibition (CDI) is a phenomenon by which bacterial cell growth is regulated by direct cell-to-cell contact via the CdiA/CdiB two-partner secretion system. Characterization of mutants resistant to CDI allowed us to identify BamA (YaeT) as the outer membrane receptor for CDI and AcrB as a potential downstream target. Notably, both BamA and AcrB are part of distinct multi-component machines. The Bam machine assembles outer membrane β-barrel proteins (OMPs) into the outer membrane and the Acr machine exports small molecules into the extracellular milieu. We discovered that a mutation that reduces expression of BamA decreased binding of CDI+ inhibitor cells, measured by flow cytometry with fluorescently-labeled bacteria. In addition, α-BamA antibodies, which recognized extracellular epitopes of BamA based on immunofluorescence, specifically blocked inhibitor-target cell binding and CDI. A second class of CDI-resistant mutants identified carried null mutations in the acrB gene. AcrB is an inner membrane component of a multidrug efflux pump that normally forms a cell envelope-spanning complex with the membrane-fusion protein AcrA and the outer membrane protein TolC. Strikingly, the requirement for the BamA and AcrB proteins in CDI is independent of their multi-component machines, and thus their role in the CDI pathway may reflect novel, import-related functions.
登录
查看更多内容
影响因子：
3.5
作者：
CHEREPANOV, PP;WACKERNAGEL, W
通讯作者：
WACKERNAGEL, W
影响因子：
3.6
作者：
BASSLER, BL;WRIGHT, M;SILVERMAN, MR
通讯作者：
SILVERMAN, MR
影响因子：
3.2
作者：
GOTTESMAN, S;TRISLER, P;TORRESCABASSA, A
通讯作者：
TORRESCABASSA, A
影响因子：
56.9
作者：
Kim, Seokhee;Malinverni, Juliana C.;Kahne, Daniel
通讯作者：
Kahne, Daniel
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--