Measuring stress signaling responses of stomata in isolated epidermis of graminaceous species.
Measuring stress signaling responses of stomata in isolated epidermis of graminaceous species.
复制标题
DOI：
10.3389/fpls.2015.00533
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Roberts MR
中科院分区：
文献类型：
--
作者：
Shen L;Sun P;Bonnell VC;Edwards KJ;Hetherington AM;McAinsh MR;Roberts MR
我们目前对保护细胞信号传导途径的理解来自于对少数模式物种的研究。研究离体表皮气孔响应的能力是阐明这些物种气孔对环境胁迫响应机制的一个重要因素。然而，这种方法很少应用于禾本科植物（包括世界上许多主要作物）气孔保护细胞信号的研究，其中保护细胞与其他植物的气孔保护细胞具有明显不同的形态。因此，我们对这些重要物种的保护细胞信号的理解要有限得多。本文介绍了大麦、小麦和短柄草背面表皮皮的分离方法。我们发现，从这些物种分离的表皮中含有可存活的保护细胞，这些细胞对脱落酸（ABA）和二氧化碳表现出典型的反应，这是通过测量气孔开度来确定的。我们使用表皮剥离试验技术更详细地研究了大麦保护细胞中不同环境因素之间的相互作用，并证明在较高温度下，气孔关闭对外部CO2的响应受到抑制，而对ABA的敏感性在30°C时比在20°C和40°C时增强。
Our current understanding of guard cell signaling pathways is derived from studies in a small number of model species. The ability to study stomatal responses in isolated epidermis has been an important factor in elucidating the mechanisms by which the stomata of these species respond to environmental stresses. However, such approaches have rarely been applied to study guard cell signaling in the stomata of graminaceous species (including many of the world’s major crops), in which the guard cells have a markedly different morphology to those in other plants. Our understanding of guard cell signaling in these important species is therefore much more limited. Here, we describe a procedure for the isolation of abaxial epidermal peels from barley, wheat and Brachypodium distachyon. We show that isolated epidermis from these species contains viable guard cells that exhibit typical responses to abscisic acid (ABA) and CO2, as determined by measurements of stomatal apertures. We use the epidermal peel assay technique to investigate in more detail interactions between different environmental factors in barley guard cells, and demonstrate that stomatal closure in response to external CO2 is inhibited at higher temperatures, whilst sensitivity to ABA is enhanced at 30°C compared to 20 and 40°C.
登录
查看更多内容
影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
影响因子：
6.9
作者：
Prokic, L;Jovanovic, Z;Stikic, R
通讯作者：
Stikic, R
影响因子：
6.9
作者：
RODRIGUEZ, JL;DAVIES, WJ
通讯作者：
DAVIES, WJ
影响因子：
5.6
作者：
Falter C;Ellinger D;von Hülsen B;Heim R;Voigt CA
通讯作者：
Voigt CA
影响因子：
7.4
作者：
Franks, Peter J.;Farquhar, Graham D.
通讯作者：
Farquhar, Graham D.