Beta class II tubulin predominates in normal and tumor breast tissues.
Beta class II tubulin predominates in normal and tumor breast tissues.
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DOI：
10.1186/bcr631
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Lobert S
中科院分区：
文献类型：
--
作者：
Dozier JH;Hiser L;Davis JA;Thomas NS;Tucci MA;Benghuzzi HA;Frankfurter A;Correia JJ;Lobert S
抗有丝分裂的化疗药物靶向有丝分裂纺锤体中的主要蛋白微管蛋白。微管蛋白同型组成被认为是肿瘤进展的诊断和细胞对化疗反应的决定因素。这表明正常组织和肿瘤组织在同型组成上存在差异。为了确定乳腺组织中是否存在这种差异，我们从配对的正常乳腺组织和肿瘤乳腺组织的分离物中分离总微管蛋白，并通过竞争性酶联免疫吸附试验（ELISA）测量β-微管蛋白I + IV、II和III类的数量。化疗前仅检查原发肿瘤组织。约30%的乳腺肿瘤发生Her2/neu蛋白扩增，被认为是预后不良的标志。为了深入了解微管蛋白同型水平是否可能与预后相关，我们使用elisa来量化这些组织中的Her2/neu蛋白水平。β-微管蛋白同型在正常乳腺组织和肿瘤乳腺组织中的分布相似。这些组织中最丰富的β-微管蛋白同型是β-微管蛋白II类和I + IV类。肿瘤组织中的Her2/neu水平是正常组织的5 - 30倍，尽管Her2/neu生物标志物与微管蛋白同型水平之间没有相关性。这些结果表明，微管蛋白同型水平，单独或联合Her2/neu蛋白水平，可能不能诊断乳腺癌的肿瘤发生。然而，这些微管蛋白同型的广泛分布（例如，40-75% β-微管蛋白II类）在乳腺组织中，结合其他因素，可能仍然与疾病进展和细胞对抗有丝分裂药物的反应有关。
Antimitotic chemotherapeutic agents target tubulin, the major protein in mitotic spindles. Tubulin isotype composition is thought to be both diagnostic of tumor progression and a determinant of the cellular response to chemotherapy. This implies that there is a difference in isotype composition between normal and tumor tissues. To determine whether such a difference occurs in breast tissues, total tubulin was fractionated from lysates of paired normal and tumor breast tissues, and the amounts of β-tubulin classes I + IV, II, and III were measured by competitive enzyme-linked immunosorbent assay (ELISA). Only primary tumor tissues, before chemotherapy, were examined. Her2/neu protein amplification occurs in about 30% of breast tumors and is considered a marker for poor prognosis. To gain insight into whether tubulin isotype levels might be correlated with prognosis, ELISAs were used to quantify Her2/neu protein levels in these tissues. β-Tubulin isotype distributions in normal and tumor breast tissues were similar. The most abundant β-tubulin isotypes in these tissues were β-tubulin classes II and I + IV. Her2/neu levels in tumor tissues were 5–30-fold those in normal tissues, although there was no correlation between the Her2/neu biomarker and tubulin isotype levels. These results suggest that tubulin isotype levels, alone or in combination with Her2/neu protein levels, might not be diagnostic of tumorigenesis in breast cancer. However, the presence of a broad distribution of these tubulin isotypes (for example, 40–75% β-tubulin class II) in breast tissue, in conjunction with other factors, might still be relevant to disease progression and cellular response to antimitotic drugs.
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DOI：
10.1073/pnas.85.12.4530
发表时间：
1988-06-01
影响因子：
11.1
作者：
HOFFMAN, PN;CLEVELAND, DW
通讯作者：
CLEVELAND, DW
影响因子：
--
作者：
GONZALEZGARAY, ML;CABRAL, F
通讯作者：
CABRAL, F
影响因子：
2.9
作者：
Derry, WB;Wilson, L;Jordan, MA
通讯作者：
Jordan, MA
影响因子：
7.5
作者：
Cassimeris, L
通讯作者：
Cassimeris, L
影响因子：
3.3
作者：
DHAMODHARAN, R;JORDAN, MA;WADSWORTH, P
通讯作者：
WADSWORTH, P