Injectable Liposome-based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs.
Injectable Liposome-based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs.
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DOI：
10.1016/j.matt.2022.03.001
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发表时间：
2022-06-01
期刊：
影响因子：
18.9
通讯作者：
Appel, Eric A.
中科院分区：
文献类型：
--
作者：
Correa, Santiago;Grosskopf, Abigail K.;Klich, John H.;Hernandez, Hector Lopez;Appel, Eric A.
指导生物功能是组织和免疫工程中下一代生物医学倡议的核心。然而，工程复杂生物网络的宏伟目标需要能够在不同的时间和地点精确地干扰特定的信号通路。利用脂质纳米技术和超分子自组装原理，我们开发了一种可注射的脂质体纳米复合水凝胶平台，以精确控制多种蛋白质药物的释放。通过将模块化脂质纳米技术集成到水凝胶中，我们引入了基于脂质体表面化学的多种释放机制。为了验证该系统用于多蛋白质递送的效用，我们证明了IgG抗体和IL-12细胞因子在体内的同步、持续和局部释放，尽管这两种蛋白质之间存在显著的尺寸差异。总的来说，脂质体水凝胶是一种高度模块化的平台技术，具有介导蛋白质释放的正交模式的能力，并具有在体外和体内精确协调生物线索的潜力。脂质体纳米复合水凝胶（LNH）是通过脂质体和改性纤维素聚合物的简单混合而开发的。这些水凝胶表现出可调的机械性能，并且易于通过标准注射器针头注射。设计脂质体表面化学使得能够独立于货物大小的蛋白质的可编程的、基于亲和力的释放。LNH可以作为体内同步释放多种治疗剂的储库。我们设想将LNH用于需要可调释放多种蛋白质药物的治疗生物医学应用。
Directing biological functions is at the heart of next-generation biomedical initiatives in tissue and immuno-engineering. However, the ambitious goal of engineering complex biological networks requires the ability to precisely perturb specific signaling pathways at distinct times and places. Using lipid nanotechnology and the principles of supramolecular self-assembly, we developed an injectable liposomal nanocomposite hydrogel platform to precisely control the release of multiple protein drugs. By integrating modular lipid nanotechnology into a hydrogel, we introduced multiple mechanisms of release based on liposome surface chemistry. To validate the utility of this system for multi-protein delivery, we demonstrated synchronized, sustained, and localized release of IgG antibody and IL-12 cytokine in vivo, despite the significant size differences between these two proteins. Overall, liposomal hydrogels are a highly modular platform technology with the ability the mediate orthogonal modes of protein release and the potential to precisely coordinate biological cues both in vitro and in vivo. Liposome Nanocomposite Hydrogels (LNHs) are developed through simple mixing of liposomes and modified cellulose polymers. These hydrogels exhibit tunable mechanical properties and are easily injected through standard syringe needles. Engineering the liposome surface chemistry enabled the programmable, affinity-based release of proteins independent of cargo size. LNHs can act as a depot to synchronously release diverse therapeutics in vivo. We envision use of LNHs for treatments biomedical applications requiring tunable release of multiple protein drugs.
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影响因子：
16.6
作者：
Appel EA;Tibbitt MW;Webber MJ;Mattix BA;Veiseh O;Langer R
通讯作者：
Langer R
影响因子：
17.1
作者：
Barberio AE;Smith SG;Correa S;Nguyen C;Nhan B;Melo M;Tokatlian T;Suh H;Irvine DJ;Hammond PT
通讯作者：
Hammond PT
DOI：
10.1111/wrr.12205
发表时间：
2014-09
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Barrientos S;Brem H;Stojadinovic O;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
9.9
作者：
Hao, T;Riman, RE
通讯作者：
Riman, RE
影响因子：
10.8
作者：
Immordino, ML;Brusa, P;Cattel, L
通讯作者：
Cattel, L