Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells.
Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells.
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源自幼稚的胚胎干细胞的猪肾类器官。
DOI：
10.3390/ijms25010682
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发表时间：
2024-01-04
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
供体肾的稀缺极大地影响了终末期肾衰竭患者的生存。猪越来越多地成为潜在的器官供体，但受到免疫排斥的限制。基于已经用CHIR 99021和FGF 9诱导策略建立的人肾类器官，我们从猪幼稚样ESC（nESC）产生了猪肾类器官。猪类器官具有管状结构和基质成分。猪类器官表达肾脏标志物，包括AQP1（近端小管）、WT1和PODO（足细胞）和CD31（血管内皮细胞）。这些结果表明，类器官已发育出大部分肾细胞类型和结构，包括肾小球和近端小管。猪类器官还被鉴定为具有葡聚糖吸收功能。重要的是，猪类器官具有一定丰度的血管内皮细胞，这是研究免疫排斥反应的基础。猪类器官可作为异种移植免疫抑制剂筛选的材料。
The scarcity of donor kidneys greatly impacts the survival of patients with end-stage renal failure. Pigs are increasingly becoming potential organ donors but are limited by immunological rejection. Based on the human kidney organoid already established with the CHIR99021 and FGF9 induction strategy, we generated porcine kidney organoids from porcine naïve-like ESCs (nESCs). The derived porcine organoids had a tubule-like constructure and matrix components. The porcine organoids expressed renal markers including AQP1 (proximal tubule), WT1 and PODO (podocyte), and CD31 (vascular endothelial cells). These results imply that the organoids had developed the majority of the renal cell types and structures, including glomeruli and proximal tubules. The porcine organoids were also identified to have a dextran absorptive function. Importantly, porcine organoids have a certain abundance of vascular endothelial cells, which are the basis for investigating immune rejection. The derived porcine organoids might serve as materials for immunosuppressor screening for xenotransplantation.
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影响因子：
6
作者：
通讯作者：
--
影响因子：
11.8
作者：
Park, Joo-Seop;Ma, Wenxiu;O'Brien, Lori L.;Chung, Eunah;Guo, Jin-Jin;Cheng, Jr-Gang;Valerius, M. Todd;McMahon, Jill A.;Wong, Wing Hung;McMahon, Andrew P.
通讯作者：
McMahon, Andrew P.
影响因子：
23.9
作者：
Low, Jian Hui;Li, Pin;Xia, Yun
通讯作者：
Xia, Yun
DOI：
10.1016/j.nefro.2016.03.023
发表时间：
2016-08-01
期刊：
Nefrología (Madrid)
影响因子：
--
作者：
González-Molina, Miguel;Ruiz-Esteban, Pedro;Hernandez, Domingo
通讯作者：
Hernandez, Domingo
影响因子：
4.6
作者：
Kumar, Santhosh, V;Er, Pei X.;Little, Melissa H.
通讯作者：
Little, Melissa H.