Characterisation of gut microbiota in Ossabaw and Göttingen minipigs as models of obesity and metabolic syndrome.
Characterisation of gut microbiota in Ossabaw and Göttingen minipigs as models of obesity and metabolic syndrome.
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DOI：
10.1371/journal.pone.0056612
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Boye M
中科院分区：
文献类型：
--
作者：
Pedersen R;Ingerslev HC;Sturek M;Alloosh M;Cirera S;Christoffersen BØ;Moesgaard SG;Larsen N;Boye M
最近的证据表明，肠道微生物群是肥胖和肥胖相关的代谢紊乱（称为代谢综合征）的重要促成因素。本研究的目的是研究两种肥胖猪模型（即Göttingen小型猪和Ossabaw小型猪）的肠道微生物群。通过基于Illumina的测序和高通量qPCR研究了来自瘦型和肥胖型Osabaw和Göttingen小型猪的盲肠、回肠和结肠微生物群，靶向不同细菌系统发育组中的16S rRNA基因。通过这项研究，肥胖的哥廷根和奥萨堡小型猪的体重增加显着。瘦型哥廷根小型猪盲肠菌群中厚壁菌门（P<0.006）、阿克曼氏菌（P<0.01）和甲烷弧菌（P<0.01）的丰度显著高于肥胖型哥廷根小型猪。肥胖的哥廷根盲肠中螺旋体门（P<0.03）、腱菌门（P<0.004）、疣微菌门（P <0.005）和拟杆菌门（P<0.001）的丰度均高于消瘦的小型猪。肥胖哥廷根小型猪的盲肠微生物群中梭菌属簇XIV的相对比例是瘦型的7.6倍。肥胖型Ossabaw小型猪回肠末端厚壁菌门丰度显著高于瘦型Ossabaw小型猪（P <0.01），结肠拟杆菌门丰度显著低于瘦型Ossabaw小型猪（P<0.01）。肥胖的奥萨鲍有显着较低的丰度普雷沃氏菌属和乳酸杆菌和较高的丰度梭菌在他们的结肠比瘦奥萨鲍。总的来说，哥廷根和奥萨堡小型猪在肠道的不同部分显示出不同的微生物群落，以应对饮食诱导的肥胖。肥胖相关的肠道微生物群组成的变化被发现在瘦与肥胖的哥廷根和Ossabaw小型猪。在这两种猪模型中，饮食似乎是塑造肠道微生物群的决定性因素，如通过瘦型和肥胖型小型猪之间不同细菌部门的变化所观察到的。
Recent evidence suggests that the gut microbiota is an important contributing factor to obesity and obesity related metabolic disorders, known as the metabolic syndrome. The aim of this study was to characterise the intestinal microbiota in two pig models of obesity namely Göttingen minipigs and the Ossabaw minipigs. The cecal, ileal and colonic microbiota from lean and obese Osabaw and Göttingen minipigs were investigated by Illumina-based sequencing and by high throughput qPCR, targeting the 16S rRNA gene in different phylogenetic groups of bacteria. The weight gain through the study was significant in obese Göttingen and Ossabaw minipigs. The lean Göttingen minipigs’ cecal microbiota contained significantly higher abundance of Firmicutes (P<0.006), Akkermensia (P<0.01) and Methanovibribacter (P<0.01) than obese Göttingen minipigs. The obese Göttingen cecum had higher abundances of the phyla Spirochaetes (P<0.03), Tenericutes (P<0.004), Verrucomicrobia (P<0.005) and the genus Bacteroides (P<0.001) compared to lean minipigs. The relative proportion of Clostridium cluster XIV was 7.6-fold higher in cecal microbiota of obese Göttingen minipigs as compared to lean. Obese Ossabaw minipigs had a higher abundance of Firmicutes in terminal ileum and lower abundance of Bacteroidetes in colon than lean Ossabaw minipigs (P<0.01). Obese Ossabaws had significantly lower abundances of the genera Prevotella and Lactobacillus and higher abundance of Clostridium in their colon than the lean Ossabaws. Overall, the Göttingen and Ossabaw minipigs displayed different microbial communities in response to diet-induced obesity in the different sections of their intestine. Obesity-related changes in the composition of the gut microbiota were found in lean versus obese Göttingen and Ossabaw minipigs. In both pig models diet seems to be the defining factor that shapes the gut microbiota as observed by changes in different bacteria divisions between lean and obese minipigs.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
3.7
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
通讯作者：
Raoult D
DOI：
10.1152/ajpendo.00352.2004
发表时间：
2005-02-01
影响因子：
5.1
作者：
Larsen, MO;Juhl, CB;Rolin, B
通讯作者：
Rolin, B
DOI：
10.1152/ajpendo.00302.2006
发表时间：
2007-03-01
影响因子：
5.1
作者：
Creely, S. J.;McTernan, P. G.;Kumar, S.
通讯作者：
Kumar, S.
影响因子：
3.7
作者：
Larsen N;Vogensen FK;van den Berg FW;Nielsen DS;Andreasen AS;Pedersen BK;Al-Soud WA;Sørensen SJ;Hansen LH;Jakobsen M
通讯作者：
Jakobsen M