Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults.
Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults.
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DOI：
10.1371/journal.pone.0009085
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发表时间：
2010-02-05
期刊：
影响因子：
3.7
通讯作者：
Jakobsen M
中科院分区：
文献类型：
--
作者：
Larsen N;Vogensen FK;van den Berg FW;Nielsen DS;Andreasen AS;Pedersen BK;Al-Soud WA;Sørensen SJ;Hansen LH;Jakobsen M
最近的证据表明，代谢性疾病和肠道中的细菌种群之间存在联系。本研究的目的是评估2型糖尿病患者和非糖尿病患者肠道微生物群组成之间的差异。该研究包括36名年龄和体重指数（BMI）范围广泛的男性成年人，其中18名被诊断为2型糖尿病。通过实时定量PCR（qPCR）研究粪便细菌组成，并在受试者亚组（N = 20）中通过16 S rRNA基因V4区的标签编码扩增子焦磷酸测序进行研究。 与对照组相比，糖尿病组中厚壁菌门和梭菌纲的比例显著降低（P = 0.03）。 此外，拟杆菌门与厚壁菌门的比例以及拟杆菌-普雷沃菌群与C.球孢E.直肠组与血糖浓度呈显著正相关（P = 0.04），与BMI无显著相关性。 类似地，与非糖尿病患者相比，糖尿病患者中β-变形菌类高度富集（P = 0.02），并且与血浆葡萄糖正相关（P = 0.04）。 这项研究的结果表明，人类2型糖尿病与肠道微生物群的组成变化有关。在将微生物群与肥胖等代谢性疾病联系起来并制定通过改变肠道微生物群来控制代谢性疾病的策略时，应考虑葡萄糖耐量水平。
Recent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim of this study was to assess the differences between the composition of the intestinal microbiota in humans with type 2 diabetes and non-diabetic persons as control. The study included 36 male adults with a broad range of age and body-mass indices (BMIs), among which 18 subjects were diagnosed with diabetes type 2. The fecal bacterial composition was investigated by real-time quantitative PCR (qPCR) and in a subgroup of subjects (N = 20) by tag-encoded amplicon pyrosequencing of the V4 region of the 16S rRNA gene. The proportions of phylum Firmicutes and class Clostridia were significantly reduced in the diabetic group compared to the control group (P = 0.03). Furthermore, the ratios of Bacteroidetes to Firmicutes as well as the ratios of Bacteroides-Prevotella group to C. coccoides-E. rectale group correlated positively and significantly with plasma glucose concentration (P = 0.04) but not with BMIs. Similarly, class Betaproteobacteria was highly enriched in diabetic compared to non-diabetic persons (P = 0.02) and positively correlated with plasma glucose (P = 0.04). The results of this study indicate that type 2 diabetes in humans is associated with compositional changes in intestinal microbiota. The level of glucose tolerance should be considered when linking microbiota with metabolic diseases such as obesity and developing strategies to control metabolic diseases by modifying the gut microbiota.
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影响因子：
3.7
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
通讯作者：
Raoult D
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM