Neutralization epitope of varicella zoster virus on native viral glycoprotein gp118 (VZV glycoprotein gpIII).
Neutralization epitope of varicella zoster virus on native viral glycoprotein gp118 (VZV glycoprotein gpIII).
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水痘带状疱疹病毒对天然病毒糖蛋白 gp118（VZV 糖蛋白 gpIII）的中和表位。
DOI：
10.1016/0042-6822(86)90124-8
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Grose,C
中科院分区：
文献类型：
--
作者：
Montalvo,EA;Grose,C
水痘-带状疱疹病毒（VZV）特异性形成几种糖蛋白，包括118，000-Da成熟结构产物（gp 118）。在产生针对低传代实验室毒株（VZV-32）和减毒疫苗毒株（VZV-Oka）的鼠单克隆抗体后，研究了gp 118的生物学和生化特性。用三种内切糖苷酶进行结构分析，（β-N-乙酰氨基葡萄糖苷酶H（内切糖苷酶H）、内切-β-N-乙酰氨基葡萄糖苷酶F（内切糖苷酶F）和内切-α-N-乙酰氨基半乳糖苷酶证明gp 118主要是一种N-连接的复合型糖蛋白，其多肽主链约为79，000 Da。但是从莫能菌素处理的感染培养物中回收的部分糖基化的中间体形式中不存在这些末端糖。与另一种VZV特异性糖蛋白gp 98不同，在加入衣霉素至VZV感染的培养物后，在gp 118上没有观察到新的寡糖部分。通过一组单克隆抗体的空斑减少试验，我们确定了该糖蛋白上的表位，该表位引起高幅度的补体非依赖性中和抗体应答。该表位是高度保守的，因为它存在于实验室VZV毒株、野生型分离株以及减毒疫苗株（VZV-Oka）上。用相同的抗gp 118单克隆抗体进行的竞争性阻断实验表明，四种中和抗体针对相似或相同的表位，而一种非中和抗体与不同的抗原位点反应。因此，本研究证明了天然病毒糖蛋白gp 118上存在免疫显性中和表位。根据新的共识命名法，这种糖蛋白将被命名为VZV gpIII。
Varicella-zoster virus (VZV) specifies the formation of several glycoproteins, including a 118,000-Da mature structural product (gp118). The biologic and biochemical properties of gp118 were studied after production of murine monoclonal antibodies to both a low-passage laboratory strain (VZV-32) and an attenuated vaccine strain (VZV-Oka). Structural analyses performed with the three glycosidases endo-(β-N-acetylglucosaminidase H (endoglycosidase H), endo-β-N-acetylglucosaminidase F (endoglycosidase F), and endo-α-N-acetylgalactosaminidase demonstrated that gp118 was predominantly an N-linked complex type glycoprotein built upon a polypeptide backbone of approximately 79,000 Da. Sialic acid residues were present on the mature glycoprotein, but these terminal sugars were absent from the partially glycosylated intermediate forms recovered from monensin-treated infected cultures. Unlike another VZV-specified glycoprotein gp98, no new oligosaccharide moieties were observed on gp118 after addition of tunicamycin to VZV-infected cultures. By plaque reduction assays with a panel of monoclonal antibodies, we defined an epitope on this glycoprotein which elicited a complement-independent neutralizing antibody response of high magnitude. The epitope was highly conserved, since it was present on a laboratory VZV strain, wild type isolates, as well as the attenuated vaccine strain (VZV-Oka). Competitive blocking experiments with the same anti-gp118 monoclonal antibodies indicated that four neutralizing antibodies were directed against similar or identical epitopes whereas one nonneutralizing antibody reacted with a different antigenic site. Thus, this study demonstrates the presence of an immunodominant neutralization epitope on native viral glycoprotein gp118. Under a new consensus nomenclature, this glycoprotein will be designated VZV gpIII.
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影响因子：
3.7
作者：
GROSE, C
通讯作者：
GROSE, C
使用细菌表达载体绘制水痘带状疱疹病毒主要糖蛋白基因 gC 的图谱
影响因子：
5.4
作者：
R. Ellis;P. Keller;R. Lowe;R. Zivin
通讯作者：
R. Zivin
DOI：
10.1073/pnas.79.15.4540
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ELDER, JH;ALEXANDER, S
通讯作者：
ALEXANDER, S
影响因子：
64.5
作者：
ROBBINS, PW;HUBBARD, SC;WIRTH, DF
通讯作者：
WIRTH, DF
影响因子：
5.4
作者：
BALACHANDRAN, N;HUTTFLETCHER, LM
通讯作者：
HUTTFLETCHER, LM