Characterization and expression of vascular endothelial growth factor (VEGF) in the ovine corpus luteum.
Characterization and expression of vascular endothelial growth factor (VEGF) in the ovine corpus luteum.
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绵羊黄体中血管内皮生长因子（VEGF）的特征和表达。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
R. Moor
中科院分区：
文献类型：
--
作者：
D. Redmer;Y. Dai;J. Li;D. Charnock;S. Smith;L. Reynolds;R. Moor
黄体在每个发情或月经周期都经历巨大的生长、发育和退化。这些变化反映在黄体血管系统同样令人印象深刻的生长和退化。我们以前已经表明，从黄体血管生成因子主要是肝素结合，这些因素之一是类似于血管内皮生长因子（VEGF）。为了鉴定该因子，并确定其在黄体血管发育中的作用，对绵羊VEGF编码区的cDNA进行了测序，并开发了一种灵敏的RNA酶保护试验，以定量发情周期早期（第2-4天）、中期（第8天）和晚期（第14-15天）母羊黄体组织中编码VEGF的mRNA。此外，从翻译的绵羊VEGF cDNA序列合成N-末端肽，并针对该肽产生抗血清用于Western免疫印迹程序。巢式逆转录酶（RT）-PCR的RNA从绵羊黄体产生三个产品，对应的大小的可变剪接的变体的VEGF（VEGF 120，VEGF 164，和VEGF 188）预测从其他物种。RNA酶保护试验显示，编码VEGF的mRNA的比例在第2-4天比第8天或第14-15天大2- 3倍。从RNase保护试验凝胶的光密度分析表明，VEGF 120代表约三分之一的总mRNA编码的VEGF在黄体，这一比例并不随发情周期的阶段。从黄体匀浆的SDS-PAGE和蛋白质免疫印迹分析显示一个单一的18 kDa的蛋白质。这些数据表明，VEGF在整个绵羊发情周期的黄体组织中表达，并且在快速黄体发育期间，当黄体血管生长达到最大值时，编码VEGF的mRNA的表达上调。
The corpus luteum undergoes tremendous growth, development and regression each oestrous or menstrual cycle. These changes are reflected by equally impressive growth and regression of the luteal vasculature. We have previously shown that angiogenic factors from corpora lutea are primarily heparin binding and that one of these factors is similar to vascular endothelial growth factor (VEGF). In an effort to identify this factor, and to define its role in luteal vascular development, the cDNA for the coding region of ovine VEGF was sequenced and a sensitive RNase protection assay was developed to quantitate mRNA encoding VEGF in luteal tissues from ewes in the early (days 2-4), mid- (day 8) and late (days 14-15) stages of the oestrous cycle. In addition, an N-terminal peptide was synthesized from the translated ovine cDNA sequence for VEGF and an antiserum was raised against this peptide for use in western immunoblotting procedures. Nested reverse transcriptase (RT)-PCR of RNA from ovine corpora lutea resulted in three products that correspond in size to the alternatively spliced variants of VEGF (VEGF120, VEGF164, and VEGF188) predicted from other species. The RNase protection assay revealed that the proportion of mRNA encoding VEGF was 2- to 3-fold greater on days 2-4 than on day 8 or days 14-15. Densitometric analysis of gels from the RNase protection assay showed that VEGF120 represented approximately one third of the total mRNA encoding VEGF in the corpus luteum and that this proportion did not vary with stage of the oestrous cycle. SDS-PAGE and western immunoblot analysis of a homogenate from corpora lutea showed a single 18 kDa protein. These data demonstrate that VEGF is expressed in luteal tissue throughout the ovine oestrous cycle and that expression of mRNA encoding VEGF is upregulated during the period of rapid luteal development, when luteal vascular growth is at its maximum.
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DOI：
10.1530/jrf.0.0910001
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
通讯作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
血管内皮生长因子信使核糖核酸在灵长类动物卵巢中的表达。
影响因子：
4.8
作者：
Ravindranath,N;Little-Ihrig,L;Phillips,HS;Ferrara,N;Zeleznik,AJ
通讯作者：
Zeleznik,AJ
DOI：
10.1016/s0021-9258(18)42003-0
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Claffey;W. Wilkison;B. Spiegelman
通讯作者：
K. Claffey;W. Wilkison;B. Spiegelman
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
B. Kamat;L. Brown;E. Manseau;D. Senger;H. Dvorak
通讯作者：
B. Kamat;L. Brown;E. Manseau;D. Senger;H. Dvorak