Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar.
Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar.
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DOI：
10.3390/ijms23094537
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发表时间：
2022-04-20
影响因子：
5.6
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中科院分区：
文献类型：
--
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木质素和纤维素是自然界中最丰富的天然有机聚合物。miRNAs是在哺乳动物、植物、病毒和细菌中发现的一类调控RNA。研究表明，miRNAs通过靶向关键酶在木质素和纤维素生物合成中发挥作用。然而，在美洲黑杨韧皮部和木质部发育中发挥作用的特异性miRNAs仍然未知。在这项研究中，通过高通量小RNA测序，共鉴定了134个miRNAs，包括132个已知的和两个新的miRNAs，其中6个仅在韧皮部中表达。在木质部和韧皮部发育过程中共发现了58个差异表达的miRNAs（DEmiRNAs）。在这些miRNA中，21个在发育中的木质部中显著上调，而韧皮部中则有37个显著下调。通过高通量degradome测序，共获得134个miRNAs的2431个靶基因。这些miRNAs的靶基因主要是转录因子，包括AP 2、ARF、bHLH、bZIP、GRAS、GRF、MYB、NAC、TCP和WRKY基因。此外，13个和9个miRNA分别参与木质素和纤维素的生物合成，我们通过qRT-PCR验证了miRNA。我们的研究探索了这些miRNAs及其在三角洲杨韧皮部和木质部发育中的调控网络，为木材形成提供了新的见解。
Lignin and cellulose are the most abundant natural organic polymers in nature. MiRNAs are a class of regulatory RNAs discovered in mammals, plants, viruses, and bacteria. Studies have shown that miRNAs play a role in lignin and cellulose biosynthesis by targeting key enzymes. However, the specific miRNAs functioning in the phloem and developing xylem of Populus deltoides are still unknown. In this study, a total of 134 miRNAs were identified via high-throughput small RNA sequencing, including 132 known and two novel miRNAs, six of which were only expressed in the phloem. A total of 58 differentially expressed miRNAs (DEmiRNAs) were identified between the developing xylem and the phloem. Among these miRNAs, 21 were significantly upregulated in the developing xylem in contrast to the phloem and 37 were significantly downregulated. A total of 2431 target genes of 134 miRNAs were obtained via high-throughput degradome sequencing. Most target genes of these miRNAs were transcription factors, including AP2, ARF, bHLH, bZIP, GRAS, GRF, MYB, NAC, TCP, and WRKY genes. Furthermore, 13 and nine miRNAs were involved in lignin and cellulose biosynthesis, respectively, and we validated the miRNAs via qRT-PCR. Our study explores these miRNAs and their regulatory networks in the phloem and developing xylem of P. deltoides and provides new insight into wood formation.
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