Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
Sperm chromatin-induced ectopic polar body extrusion in mouse eggs after ICSI and delayed egg activation.
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DOI：
10.1371/journal.pone.0007171
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发表时间：
2009-09-29
期刊：
影响因子：
3.7
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Deng M;Li R
卵母细胞内的减数分裂染色体不仅是母体基因组的载体，而且提供位置信号，诱导卵母细胞皮质极化，决定卵母细胞的非对称减数分裂，导致母体基因组极体挤压和单倍体化。减数分裂染色体在决定减数分裂中起双重作用：1)组织两极纺锤体形成；2)诱导皮层极化，并在上覆皮层中组装独特的皮质细胞骨架结构，用于极体挤压。受精时，精子将外源的父本染色质带入卵子，在精子进入部位诱发异位皮质极化，并导致锥体形成，称为受精锥体。本研究表明，精子染色质诱导的受精锥体形成是由于精子染色质在受精过程中缺乏纺锤体诱导而导致的流产极体挤压。如果通过实验操纵受精过程，使精子染色质同时诱导皮层极化和纺锤体形成，则受精锥可以转化为极体挤压。这表明精子染色质也能诱导极体挤压，就像它的母体一样。受精过程中通常观察到的锥体形成，而不是由精子染色质引起的异位极体挤压，这是由于精子染色质的特殊压实抑制了它的快速纺锤体诱导，因此提供了一种保护机制，以防止在异位极体挤压过程中可能出现的父本基因组丢失。
Meiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play dual function in determination of meiosis: 1) organizing a bipolar spindle formation and 2) inducing cortical polarization and assembly of a distinct cortical cytoskeleton structure in the overlying cortex for polar body extrusion. At fertilization, a sperm brings exogenous paternal chromatin into the egg, which induces ectopic cortical polarization at the sperm entry site and leads to a cone formation, known as fertilization cone. Here we show that the sperm chromatin-induced fertilization cone formation is an abortive polar body extrusion due to lack of spindle induction by the sperm chromatin during fertilization. If experimentally manipulating the fertilization process to allow sperm chromatin to induce both cortical polarization and spindle formation, the fertilization cone can be converted into polar body extrusion. This suggests that sperm chromatin is also able to induce polar body extrusion, like its maternal counterpart. The usually observed cone formation instead of ectopic polar body extrusion induced by sperm chromatin during fertilization is due to special sperm chromatin compaction which restrains it from rapid spindle induction and therefore provides a protective mechanism to prevent a possible paternal genome loss during ectopic polar body extrusion.
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DOI：
10.1002/mrd.1120190308
发表时间：
1988-03-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
CROZET, N
通讯作者：
CROZET, N
影响因子：
3.6
作者：
Cho, C;Jung-Ha, H;Eddy, EM
通讯作者：
Eddy, EM
影响因子：
64.8
作者：
Heald, R;Tournebize, R;Karsenti, E
通讯作者：
Karsenti, E
影响因子：
11.8
作者：
Deng, Manqi;Suraneni, Praveen;Li, Rong
通讯作者：
Li, Rong
影响因子：
2.7
作者：
Ducibella, T;Huneau, D;Ozil, JP
通讯作者：
Ozil, JP