Transcriptome Analysis of Rice Seedling Roots in Response to Potassium Deficiency.
Transcriptome Analysis of Rice Seedling Roots in Response to Potassium Deficiency.
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DOI：
10.1038/s41598-017-05887-9
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Xue D
中科院分区：
文献类型：
--
作者：
Zhang X;Jiang H;Wang H;Cui J;Wang J;Hu J;Guo L;Qian Q;Xue D
水稻是世界上最重要的粮食作物之一，其生长、发育、产量和品质易受大量营养素钾（K+）缺乏的影响。耐K+缺乏的分子机制仍知之甚少。在这项研究中，K+缺乏的条件下，水稻幼苗根系转录组的变化进行了调查。利用核糖核酸测序（RNA-Seq）分析，共获得805个差异表达基因，其中上调基因536个，下调基因269个。基因功能分类表明，参与营养物质运输，蛋白激酶，转录过程和植物激素的基因的表达，特别是在根中改变。虽然这些变化是显着的，大多数基因的表达保持不变，即使在K+缺乏的条件下。有趣的是，当我们的RNA-Seq结果与公开的微阵列数据进行比较时，我们发现大多数在低K+条件下差异表达的基因在其他环境胁迫条件下也表现出表达变化。
Rice is one of the most important food crops in the world, and its growth, development, yield, and grain quality are susceptible to a deficiency of the macronutrient potassium (K+). The molecular mechanism for K+ deficiency tolerance remains poorly understood. In this study, K+ deficient conditions were employed to investigate the resulting changes in the transcriptome of rice seedling roots. Using ribonucleic acid sequencing (RNA-Seq) and analysis, a total of 805 differentially expressed genes were obtained, of which 536 genes were upregulated and 269 were downregulated. Gene functional classification showed that the expression of genes involved in nutrient transport, protein kinases, transcription processes, and plant hormones were particularly altered in the roots. Although these changes were significant, the expression of most genes remained constant even in K+-deficient conditions. Interestingly, when our RNA-Seq results were compared to public microarray data, we found that most of the genes that were differentially expressed in low K+ conditions also exhibited changes in expression in other environmental stress conditions.
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